Document Detail

Modification of the oxygen affinity and intracellular hemoglobin concentration of normal and sickle cells by means of an osmotic pulse.
MedLine Citation:
PMID:  2909651     Owner:  NLM     Status:  MEDLINE    
Important properties of red blood cells (RBCs), including oxygen affinity and intracellular hemoglobin concentration, may be modified by means of an osmotic pulse. The pulse is developed by rapid dilution of a suspension of RBCs to which dimethylsulfoxide has been added. The nature and degree of RBC modification is dependent on the composition of the diluent solution. The purpose of this investigation was to explore the dependence of these changes on diluent composition and to determine the stability of altered cellular properties with in vitro incubation. For both normal control RBCs (A cells) and RBCs from patients with sickle cell anemia (S cells), cells with increased volume and markedly decreased intracellular hemoglobin concentration (65% to 70% of control) or with normal volume and moderately decreased hemoglobin concentration (80% to 85% of control) were prepared. These modifications were accomplished with hemoglobin yields ranging from 60% to 90%, depending on the diluent used, the cell type, and the intensity of treatment. For the same degree of inositol hexaphosphate (IHP) incorporation into A cells, as shown by the shift in oxygen half-saturation pressure (P50), diluent formulations with constant total osmolality but varying IHP concentration gave the same degree of hemoglobin loss per cell. Posttreatment cell size, however, ranged from slightly smaller than control to approximately 20% larger. On incubation, the larger cells returned toward normal size, whereas those with normal posttreatment size remained constant. S cells showed the same general changes with treatment and incubation but with greater variation. The treated S cells exhibited markedly reduced morphologic sickling on deoxygenation.
R S Franco; R L Barker
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  113     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-02-09     Completed Date:  1989-02-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  58-66     Citation Subset:  AIM; IM    
Department of Internal Medicine, University of Cincinnati College of Medicine, OH.
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MeSH Terms
Adenosine Triphosphate / blood
Anemia, Sickle Cell / blood*
Erythrocyte Indices
Erythrocytes / analysis*
Hemoglobins / analysis*
Osmotic Pressure
Oxygen / blood*
Reg. No./Substance:
0/Hemoglobins; 56-65-5/Adenosine Triphosphate; 7782-44-7/Oxygen

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