Document Detail


Modification of mipafox-induced inhibition of neuropathy target esterase in neuroblastoma cells of human origin.
MedLine Citation:
PMID:  8337699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A neuroblastoma cell line of human origin was used as an in vitro model system to examine early effects on inhibition of neuropathy target esterase (NTE, also known as neurotoxic esterase) in the presence of agents belonging to classes of chemicals previously demonstrated to modify organophosphorus-induced delayed neuropathy in hens. For this study, differentiated SY-5Y cells were treated for up to 10 min with mipafox, an organophosphorus compound, and NTE inhibition was determined when cells exposed to mipafox were also exposed to the carbamate, aldicarb, and to the calcium channel blocker, verapamil. Cells were exposed to aldicarb or verapamil 5 min before, at the same time, or 2 min after mipafox. Less NTE inhibition was observed when either aldicarb or verapamil was included in the incubation of SY-5Y cells with mipafox. Effects of aldicarb and verapamil on NTE inhibition in differentiated SY-5Y cells were similar to effects in chicken brain homogenates. These results indicate that NTE inhibition can be detected in neuroblastoma cells, that these cells respond in a manner similar to chicken brain, and that mipafox-induced inhibition of NTE can be decreased in the presence of aldicarb or verapamil.
Authors:
A C Nostrandt; M Ehrich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  121     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-08-20     Completed Date:  1993-08-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  36-42     Citation Subset:  IM    
Affiliation:
Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0442.
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MeSH Terms
Descriptor/Qualifier:
Aldicarb / pharmacology*
Animals
Brain / drug effects,  enzymology
Carboxylic Ester Hydrolases / antagonists & inhibitors*
Chickens
Humans
Isoflurophate / analogs & derivatives*,  toxicity
Nerve Growth Factors / pharmacology
Neuroblastoma / enzymology*,  pathology
Tumor Cells, Cultured
Verapamil / pharmacology*
Chemical
Reg. No./Substance:
0/Nerve Growth Factors; 116-06-3/Aldicarb; 371-86-8/mipafox; 52-53-9/Verapamil; 55-91-4/Isoflurophate; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/neurotoxic esterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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