| Modification of lipopolysaccharide non-responder cells in low cell density culture. | |
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MedLine Citation:
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PMID: 6195102 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipopolysaccharide (LPS) fails to induce polyclonal antibody responses in LPS non-responder C3H/HeJ mice in vivo and also in in vitro cultures of 10(7) cells/ml of C3H/HeJ spleen cells. However, this study shows that LPS elicits polyclonal antibody synthesis in low cell density cultures of C3H/HeJ cells by activation of a translational process without an increase in total RNA and messenger RNA synthesis. Polyclonal antibody synthesis by LPS non-responder cells in low cell density culture was significantly increased by LPS in either serum-free medium or medium containing serum. LPS-induced antibody production by LPS non-responder cells in low cell density was not due to the relative elevation of LPS concentrations. |
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Authors:
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S Ishizaka |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Immunology letters Volume: 6 ISSN: 0165-2478 ISO Abbreviation: Immunol. Lett. Publication Date: 1983 Jun |
Date Detail:
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Created Date: 1983-12-17 Completed Date: 1983-12-17 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7910006 Medline TA: Immunol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 343-50 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibody Formation* Cell Count Cells, Cultured Immunoglobulin G / biosynthesis Immunoglobulin M / biosynthesis Lipopolysaccharides / pharmacology* Lymphocytes / immunology*, metabolism Mice Mice, Inbred C3H Polyribosomes / metabolism Protein Biosynthesis RNA / biosynthesis Spleen / cytology |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; 0/Immunoglobulin M; 0/Lipopolysaccharides; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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