| Modification of glycopeptidolipids by an O-methyltransferase of Mycobacterium smegmatis. | |
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MedLine Citation:
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PMID: 12368441 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycopeptidolipids (GPLs) are a major component of the outer layers of the cell walls of several non-tuberculous mycobacteria. The Mycobacterium smegmatis GPLs consist of a diglycosylated lipopeptide core which is variably modified by acetylation and methylation. Analysis of a region of the M. smegmatis chromosome, upstream of the peptide synthetase gene, mps, revealed a GPL biosynthetic locus containing genes potentially involved in glycosylation, methylation, acetylation and transport of GPLs. Methyltransferases are required to modify rhamnose and the fatty acid of GPLs. Of the four methyltransferases encoded within the locus, one methyltransferase, Mtf2, was unlike sugar methyltransferases from other species. An mtf2 mutant was created and was shown to be unable to methylate the GPL fatty acids. Direct evidence is presented that Mtf2 is a methyltransferase that modifies the GPL fatty acid. |
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Authors:
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Dharshini Jeevarajah; John H Patterson; Malcolm J McConville; Helen Billman-Jacobe |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 148 ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-10-07 Completed Date: 2003-01-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
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Languages: eng Pagination: 3079-87 Citation Subset: IM |
Affiliation:
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Departments of Microbiology and Immunology and Biochemistry and Molecular Biology, University of Melbourne, Victoria 3010, Australia. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY138899 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Amino Acid Sequence Bacterial Proteins / genetics, metabolism Gas Chromatography-Mass Spectrometry Gene Deletion Genetic Complementation Test Glycolipids / chemistry, metabolism* Glycopeptides / chemistry, metabolism* Methylation Methyltransferases / genetics, metabolism* Molecular Sequence Data Multigene Family* Mycobacterium smegmatis / enzymology*, genetics Sequence Analysis, DNA Spectrometry, Mass, Electrospray Ionization |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Glycolipids; 0/Glycopeptides; EC 2.1.1.-/Methyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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