Document Detail

Modification of glycopeptidolipids by an O-methyltransferase of Mycobacterium smegmatis.
MedLine Citation:
PMID:  12368441     Owner:  NLM     Status:  MEDLINE    
Glycopeptidolipids (GPLs) are a major component of the outer layers of the cell walls of several non-tuberculous mycobacteria. The Mycobacterium smegmatis GPLs consist of a diglycosylated lipopeptide core which is variably modified by acetylation and methylation. Analysis of a region of the M. smegmatis chromosome, upstream of the peptide synthetase gene, mps, revealed a GPL biosynthetic locus containing genes potentially involved in glycosylation, methylation, acetylation and transport of GPLs. Methyltransferases are required to modify rhamnose and the fatty acid of GPLs. Of the four methyltransferases encoded within the locus, one methyltransferase, Mtf2, was unlike sugar methyltransferases from other species. An mtf2 mutant was created and was shown to be unable to methylate the GPL fatty acids. Direct evidence is presented that Mtf2 is a methyltransferase that modifies the GPL fatty acid.
Dharshini Jeevarajah; John H Patterson; Malcolm J McConville; Helen Billman-Jacobe
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  148     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-07     Completed Date:  2003-01-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  3079-87     Citation Subset:  IM    
Departments of Microbiology and Immunology and Biochemistry and Molecular Biology, University of Melbourne, Victoria 3010, Australia.
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MeSH Terms
Amino Acid Motifs
Amino Acid Sequence
Bacterial Proteins / genetics,  metabolism
Gas Chromatography-Mass Spectrometry
Gene Deletion
Genetic Complementation Test
Glycolipids / chemistry,  metabolism*
Glycopeptides / chemistry,  metabolism*
Methyltransferases / genetics,  metabolism*
Molecular Sequence Data
Multigene Family*
Mycobacterium smegmatis / enzymology*,  genetics
Sequence Analysis, DNA
Spectrometry, Mass, Electrospray Ionization
Reg. No./Substance:
0/Bacterial Proteins; 0/Glycolipids; 0/Glycopeptides; EC 2.1.1.-/Methyltransferases

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