| Modification of gibberellin signalling (metabolism & signal transduction) in sugar beet: analysis of potential targets for crop improvement. | |
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MedLine Citation:
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PMID: 18696248 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sugar beet, Beta vulgaris spp. vulgaris is a biennial long day plant with an obligate requirement for vernalization (prolonged exposure to low temperature). As a spring crop in temperate European climates, it is vulnerable to vernalization-induced premature bolting and flowering, resulting in reduced crop yield and quality. Gibberellins (GAs) play important roles in key physiological processes including stem elongation (bolting) and flowering and are, therefore, potential targets for controlling reproductive growth in sugar beet. We show that the BvGA20ox gene, which encodes an enzyme necessary for GA biosynthesis, was transcriptionally activated in apices of sugar beet plants after vernalization and that GA metabolism can be manipulated to delay bolting in vernalized plants. We demonstrate that down-regulation of GA responses by transformation with the Arabidopsis thaliana gai gene (which represses GA signalling), under its own promoter (pgai::gai) or deactivation of GA by over-expression of the Phaseolus coccineus (bean) GA2ox1 gene, which inactivates GA, increased the required post vernalization thermal time (an accurate and stable measure of physiological time), to bolt by approximately 300 degrees Cd. This resulted in agronomically significant bolting time delays of approximately 2 weeks and 3 weeks in the pgai::gai and 35S::PcGA2ox1 plants, respectively. Our data represent the first transgenic sugar beet model to (1) show that GA signalling can be used to improve crops by manipulation of the transition to reproductive growth; and (2) provide evidence that GA is required for seed set in sugar beet. |
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Authors:
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Effie Mutasa-Gottgens; Aiming Qi; Ann Mathews; Stephen Thomas; Andrew Phillips; Peter Hedden |
Publication Detail:
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Type: Journal Article Date: 2008-08-12 |
Journal Detail:
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Title: Transgenic research Volume: 18 ISSN: 0962-8819 ISO Abbreviation: Transgenic Res. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-11 Completed Date: 2009-07-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9209120 Medline TA: Transgenic Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 301-8 Citation Subset: IM |
Affiliation:
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Broom's Barn Research Station, Higham, Bury St. Edmunds, Suffolk IP28 6NP, UK. effie.mutasa@bbsrc.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics Arabidopsis Proteins / genetics Beta vulgaris / genetics*, metabolism* Crops, Agricultural / metabolism* Gene Expression Regulation, Plant Genetic Techniques Gibberellins / metabolism* Light Models, Genetic Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic Signal Transduction Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/GAI protein, Arabidopsis; 0/Gibberellins; 0/Plant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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