Document Detail


Modification of an existing chromosomal inversion to engineer a balancer for mouse chromosome 15.
MedLine Citation:
PMID:  15238537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosomal inversions are valuable genetic tools for mutagenesis screens, where appropriately marked inversions can be used as balancer chromosomes to recover and maintain mutations in the corresponding chromosomal region. For any inversion to be effective as a balancer, it should exhibit both dominant and recessive visible traits; ideally the recessive trait should be a fully penetrant lethality in which inversion homozygotes die before birth. Unfortunately, most inversions recovered by classical radiation or chemical mutagenesis techniques do not have an overt phenotype in either the heterozygous or the homozygous state. However, they can be modified by relatively simple procedures to make them suitable as an appropriately marked balancer. We have used homologous recombination to modify, in embryonic stem cells, the recessive-lethal In(15)21Rk inversion to endow it with a dominant-visible phenotype. Several ES cell lines were derived from inversion heterozygotes, and a keratin-14 (K14) promoter-driven agouti minigene was introduced onto the inverted chromosome 15 in the ES cells by gene targeting. Mice derived from the targeted ES cells carry the inverted chromosome 15 and, at the same time, exhibit lighter coat color on their ears and tails, making this modified In(15)21Rk useful as a balancer for proximal mouse chromosome 15.
Authors:
Wallace S H Chick; Sarah E Mentzer; Donald A Carpenter; Eugene M Rinchik; Yun You
Related Documents :
16721377 - Assaying chromosomal inversions by single-molecule haplotyping.
20974007 - Breakpoint structure of the anopheles gambiae 2rb chromosomal inversion.
7069187 - Cladistic analysis of mosquito chromosome data (anopheles (cellia) myzomyia.
17329177 - A fourteen years follow-up of a case of partial trisomy 12q and monosomy 12p recombinan...
18408757 - Unbalanced translocation, a major chromosome alteration causing loss of heterozygosity ...
1765157 - Compositional properties of telomeric regions from human chromosomes.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  167     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-07-07     Completed Date:  2005-04-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  889-95     Citation Subset:  IM    
Affiliation:
Graduate School of Genome Sciences and Technology, Cellular and Molecular Biology, The University of Tennessee, Knoxville, 37996, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Inversion / genetics*
Chromosome Mapping*
Exons / genetics
Genetic Engineering
Heterozygote
Homozygote
Mice
Mutagenesis
Mutagenesis, Insertional
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Rapid evolution of mammalian X-linked testis-expressed homeobox genes.
Next Document:  Paternal mitochondrial DNA transmission during nonhuman primate nuclear transfer.