Document Detail

Modification of effects of radiation on thymidine kinase.
MedLine Citation:
PMID:  1973443     Owner:  NLM     Status:  MEDLINE    
Thymidine kinase (TdR-K) and the incorporation of iododeoxyuridine (IUdR) into DNA of murine bone marrow cells are acutely and temporarily inhibited by low doses (0.01 Gy) of whole-body gamma-radiation with a maximal effect at 4 h after exposure and full recovery at 10 h. The inhibitory effect was totally abolished by whole-body exposure to a strong static magnetic field of 1.4T. The present investigation was designed to gain insight into the mechanism(s) underlying the inhibition of TdR-K activity and the incorporation of 125I-UdR by challenging the system with various pharmacological and biochemical means. To this end the response of TdR-K and 125I-UdR incorporation into DNA to the administration of actinomycin-D, cycloheximide, cysteamine, misonidazole and procaine hydrochloride as well as to dietary manipulations, i.e. vitamin E deficiency and enrichment of the diet by soya oil, and to changes in the glutathione levels were investigated in bone marrow cells of irradiated and sham-irradiated mice. Furthermore, the effect of various NaHCO3 concentrations on optimizing the radiation-induced inhibition of TdR-K was investigated under conditions of radiation, vitamin E deficiency and enrichment of the diet by soya oil. The data point to the involvement of the cellular radical-detoxification system in changing the activity of TdR-K, especially on the basis of the concurrent increase of glutathione concentration and decrease in TdR-K activity.
K Hohn-Elkarim; H Mühlensiepen; K I Altman; L E Feinendegen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  58     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-08-23     Completed Date:  1990-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  97-110     Citation Subset:  IM; S    
Institute for Medicine, Nuclear Research Center Jülich GmbH, FRG.
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MeSH Terms
Bone Marrow / metabolism
Cesium Radioisotopes
DNA / metabolism
Gamma Rays
Idoxuridine / pharmacokinetics
Thymidine Kinase / metabolism,  radiation effects*
Whole-Body Irradiation*
Reg. No./Substance:
0/Cesium Radioisotopes; 54-42-2/Idoxuridine; 9007-49-2/DNA; EC Kinase

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