Document Detail


Modification of bone graft by blending with lecithin to improve hydrophilicity and biocompatibility.
MedLine Citation:
PMID:  18458499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lecithin was blended to improve the hydrophilicity and biocompatibility of bone graft containing poly(l-lactic acid) (PLLA). Solution blending and freeze drying were used to fabricate symmetrical scaffolds containing different percentages of lecithin (lecithin: PLLA = 0, 5, 10 wt%). Scanning electron microscopy showed that the scaffolds maintained the three-dimensional porous structure. A water uptake experiment proved the significant improvement of hydrophilicity of the blend scaffold. With the addition of lecithin, the compressive strength and compressive modulus decreased. When the weight ratio of lecithin to PLLA was up to 10%, the compressive strength was still more than the lower limit of natural cancellous bone. To test the biocompatibility of the scaffolds, cell culture in vitro and subcutaneous implantation in vivo were performed. MC3T3-E1 preosteoblastic cells were cultured on the scaffolds for 7 days. Methylthiazol tetrazolium assay and laser scanning confocal microscopy were used to exhibit proliferation and morphology of the cells. The subcutaneous implantation in rats tested inflammatory response to the scaffolds. The results proved the better biocompatibility and milder inflammatory reactions of the blend scaffold (lecithin: PLLA = 5%) compared with the scaffold without lecithin. The modified scaffold containing lecithin is promising for bone tissue engineering.
Authors:
Y Wang; F Z Cui; Y P Jiao; K Hu; D D Fan
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-03
Journal Detail:
Title:  Biomedical materials (Bristol, England)     Volume:  3     ISSN:  1748-605X     ISO Abbreviation:  Biomed Mater     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-05-06     Completed Date:  2008-06-04     Revised Date:  2010-03-11    
Medline Journal Info:
Nlm Unique ID:  101285195     Medline TA:  Biomed Mater     Country:  England    
Other Details:
Languages:  eng     Pagination:  015012     Citation Subset:  IM    
Affiliation:
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Bone Substitutes / administration & dosage*,  chemistry*
Bone Transplantation / methods*
Cell Proliferation / drug effects*
Hydrophobicity
Lactic Acid / administration & dosage*,  chemistry*
Lecithins / administration & dosage*,  chemistry*
Materials Testing
Mice
Polymers / administration & dosage*,  chemistry*
Rats
Chemical
Reg. No./Substance:
0/Bone Substitutes; 0/Lecithins; 0/Polymers; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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