Document Detail

Modification of HT 29 cell response to the vasoactive intestinal peptide (VIP) by membrane fluidization.
MedLine Citation:
PMID:  3006797     Owner:  NLM     Status:  MEDLINE    
We used liposomes made with phospholipids of fatty acid chain length ranging from C12:0 to C16:0 to modify the cAMP dependent protein kinase (PK) activity of HT 29 cells induced by VIP or forskolin. Both VIP and forskolin effects were inhibited in dilauroylphosphatidylcholine (DLPC) treated cells. PK activity was slightly lowered when cells were treated by dimyristoylphosphatidylcholine (DMPC) liposomes. However neither VIP nor forskolin-induced PK activities were affected with dipalmitoylphosphatidylcholine (DPPC) liposomes. Furthermore, the binding of [125I]VIP to DLPC treated cells was drastically lowered whereas no change was observed when cells were incubated with DMPC or DPPC liposomes. On the other hand, the interaction of HT 29 cells with DLPC vesicles provoked a decrease in membrane cholesterol content with subsequent increase in membrane fluidity. These findings provide evidence that, in HT 29 cells, the mechanisms of VIP-receptor interaction and of adenylate cyclase activation is lipid dependent and is regulated by membrane fluidity.
A el Battari; E Ah-Kye; J M Muller; H Sari; J Marvaldi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  67     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-04-25     Completed Date:  1986-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  1217-23     Citation Subset:  IM    
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MeSH Terms
Cell Line
Colonic Neoplasms
Membrane Fluidity / drug effects*
Phosphatidic Acids
Receptors, Cell Surface / metabolism
Receptors, Vasoactive Intestinal Peptide
Vasoactive Intestinal Peptide / metabolism,  pharmacology*
Reg. No./Substance:
0/Liposomes; 0/Lysophosphatidylcholines; 0/Phosphatidic Acids; 0/Receptors, Cell Surface; 0/Receptors, Vasoactive Intestinal Peptide; 37221-79-7/Vasoactive Intestinal Peptide

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