| Modification of a French pressure cell to improve microbial cell disruption. | |
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MedLine Citation:
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PMID: 19731071 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A procedure for modification of the valve stem of a 40 K French pressure cell is described. The modification should be done by a machinist and requires a metalworking lathe. After modification of the valve stem, a torlon 4203 plastic ball is used between the valve stem and valve seat to control the pressure within the cell. The torlon plastic ball is a key component needed to obtain the high pressures required for efficient disruption of microbial cells. |
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Authors:
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Paul R Jaschke; Ian Drake; J Thomas Beatty |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Photosynthesis research Volume: 102 ISSN: 1573-5079 ISO Abbreviation: Photosyn. Res. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-07 Completed Date: 2010-02-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100954728 Medline TA: Photosynth Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 95-7 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Fractionation
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instrumentation* Rhodobacter sphaeroides / cytology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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