| Modern tools for identification of nucleic acid-binding proteins. | |
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MedLine Citation:
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PMID: 18452716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Numerous biological mechanisms depend on nucleic acid--protein interactions. The first step to the understanding of these mechanisms is to identify interacting molecules. Knowing one partner, the identification of other associated molecular species can be carried out using affinity-based purification procedures. When the nucleic acid-binding protein is known, the nucleic acid can be isolated and identified by sensitive techniques such as polymerase chain reaction followed by DNA sequencing or hybridization on chips. The reverse identification procedure is less straightforward in part because interesting nucleic acid-binding proteins are generally of low abundance and there are no methods to amplify amino acid sequences. In this article, we will review the strategies that have been developed to identify nucleic acid-binding proteins. We will focus on methods permitting the identification of these proteins without a priori knowledge of protein candidates. |
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Authors:
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Nadia Hégarat; Jean-Christophe François; Danièle Praseuth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2008-04-12 |
Journal Detail:
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Title: Biochimie Volume: 90 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-07-29 Completed Date: 2008-10-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 1265-72 Citation Subset: IM |
Affiliation:
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INSERM, U565 Case Postale 26, 57 rue Cuvier, 75231 Paris Cedex 05, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA-Binding Proteins / chemistry*, genetics, metabolism* Databases, Protein Humans Microarray Analysis Nucleic Acids / chemistry*, metabolism* Protein Binding RNA-Binding Proteins / chemistry*, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Nucleic Acids; 0/RNA-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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