Document Detail


Moderate exercise improves leucocyte function and decreases inflammation in diabetes.
MedLine Citation:
PMID:  20846161     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The genesis and progression of diabetes occur due in part to an uncontrolled inflammation profile with insulin resistance, increased serum levels of free fatty acids (FFA), proinflammatory cytokines and leucocyte dysfunction. In this study, an investigation was made of the effect of a 3-week moderate exercise regimen on a treadmill (60% of VO₂(max) , 30 min/day, 6 days a week) on inflammatory markers and leucocyte functions in diabetic rats. The exercise decreased serum levels of tumour necrosis factor (TNF)-α (6%), cytokine-induced neutrophil chemotactic factor 2 alpha/beta (CINC-2α/β) (9%), interleukin (IL)-1β (34%), IL-6 (86%), C-reactive protein (CRP) (41%) and FFA (40%) in diabetic rats when compared with sedentary diabetic animals. Exercise also attenuated the increased responsiveness of leucocytes from diabetics when compared to controls, diminishing the reactive oxygen species (ROS) release by neutrophils (21%) and macrophages (28%). Exercise did not change neutrophil migration and the proportion of neutrophils and macrophages in necrosis (loss of plasma membrane integrity) and apoptosis (DNA fragmentation). Serum activities of creatine kinase (CK) and lactate dehydrogenase (LDH) were not modified in the conditions studied. Therefore, physical training did not alter the integrity of muscle cells. We conclude that moderate physical exercise has marked anti-inflammatory effects on diabetic rats. This may be an efficient strategy to protect diabetics against microorganism infection, insulin resistance and vascular complications.
Authors:
M F Belotto; J Magdalon; H G Rodrigues; M A R Vinolo; R Curi; T C Pithon-Curi; E Hatanaka
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-15
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  162     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-02     Completed Date:  2011-01-10     Revised Date:  2011-11-01    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  237-43     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Clinical and Experimental Immunology © 2010 British Society for Immunology.
Affiliation:
Cruzeiro do Sul University, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / immunology
C-Reactive Protein / metabolism
Chemokines, CXC / blood
Creatine Kinase / blood
Cytokines / blood
Diabetes Mellitus, Experimental / blood,  immunology*,  therapy
Diabetes Mellitus, Type 1 / blood,  immunology,  therapy
Fatty Acids, Nonesterified / blood
Inflammation / blood,  immunology
Interleukins / blood
L-Lactate Dehydrogenase / blood
Leukocytes / immunology*,  metabolism
Macrophages, Peritoneal / cytology,  drug effects,  metabolism,  pathology
Male
Necrosis / immunology,  pathology
Neutrophils / cytology,  drug effects,  metabolism,  pathology
Peritoneal Cavity / cytology
Physical Conditioning, Animal / physiology*
Rats
Rats, Wistar
Reactive Oxygen Species / metabolism
Tetradecanoylphorbol Acetate / pharmacology
Tumor Necrosis Factor-alpha / blood
Chemical
Reg. No./Substance:
0/Chemokines, CXC; 0/Cytokines; 0/Fatty Acids, Nonesterified; 0/Gm1960 protein, rat; 0/Interleukins; 0/Reactive Oxygen Species; 0/Tumor Necrosis Factor-alpha; 16561-29-8/Tetradecanoylphorbol Acetate; 9007-41-4/C-Reactive Protein; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.3.2/Creatine Kinase

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