| Modelling the peroxisomal carbon leak during lipid mobilization in Arabidopsis. | |
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MedLine Citation:
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PMID: 20863290 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mutation of the ACN1 (acetate non-utilizing 1) locus of Arabidopsis results in altered acetate assimilation into gluconeogenic sugars and anapleurotic amino acids and leads to an overall depression in primary metabolite levels by approx. 50% during seedling development. Levels of acetyl-CoA were higher in acn1 compared with wild-type, which is counterintuitive to the activity of ACN1 as a peroxisomal acetyl-CoA synthetase. We hypothesize that ACN1 recycles free acetate to acetyl-CoA within peroxisomes in order that carbon remains fed into the glyoxylate cycle. When ACN1 is not present, carbon in the form of acetate can leak out of peroxisomes and is reactivated to acetyl-CoA within the cytosol. Kinetic models incorporating estimates of carbon input and pathway dynamics from a variety of literature sources have proven useful in explaining how ACN1 may prevent the carbon leak and even contribute to the control of peroxisomal carbon metabolism. |
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Authors:
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Mark A Hooks; Elizabeth Allen; Jonathan A D Wattis |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical Society transactions Volume: 38 ISSN: 1470-8752 ISO Abbreviation: Biochem. Soc. Trans. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-24 Completed Date: 2011-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7506897 Medline TA: Biochem Soc Trans Country: England |
Other Details:
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Languages: eng Pagination: 1230-3 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, UK. m.a.hooks@bangor.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Acetyl Coenzyme A / metabolism Arabidopsis / genetics, metabolism* Arabidopsis Proteins / genetics, metabolism Carbon / metabolism* Coenzyme A Ligases / genetics, metabolism Lipid Metabolism Peroxisomes / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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P14659//Biotechnology and Biological Sciences Research Council; P19408//Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Arabidopsis Proteins; 72-89-9/Acetyl Coenzyme A; 7440-44-0/Carbon; EC 6.2.1.-/Coenzyme A Ligases; EC 6.2.1.13/acetate-CoA ligase (ADP-forming) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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