Document Detail

Modelling biological cell attachment and growth on adherent surfaces.
MedLine Citation:
PMID:  23412665     Owner:  NLM     Status:  Publisher    
A mathematical model, in the form of an integro-partial differential equation, is presented to describe the dynamics of cells being deposited, attaching and growing in the form of a monolayer across an adherent surface. The model takes into account that the cells suspended in the media used for the seeding have a distribution of sizes, and that the attachment of cells restricts further deposition by fragmenting the parts of the domain unoccupied by cells. Once attached the cells are assumed to be able to grow and proliferate over the domain by a process of infilling of the interstitial gaps; it is shown that without cell proliferation there is a slow build up of the monolayer but if the surface is conducive to cell spreading and proliferation then complete coverage of the domain by the monolayer can be achieved more rapidly. Analytical solutions of the model equations are obtained for special cases, and numerical solutions are presented for parameter values derived from experiments of rat mesenchymal stromal cells seeded onto thin layers of collagen-coated polyethylene terephthalate electrospun fibers. The model represents a new approach to describing the deposition, attachment and growth of cells over adherent surfaces, and should prove useful for studying the dynamics of the seeding of biomaterials.
Greg Lemon; Ylva Gustafsson; Johannes C Haag; Mei L Lim; Sebastian Sjöqvist; Fatemeh Ajalloueian; Philipp Jungebluth; Paolo Macchiarini
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-15
Journal Detail:
Title:  Journal of mathematical biology     Volume:  -     ISSN:  1432-1416     ISO Abbreviation:  J Math Biol     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502105     Medline TA:  J Math Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Advanced Center for Translational Regenerative Medicine (ACTREM), Karolinska Institutet, Alfred Nobels Allé 8, 141 86 , Huddinge, Sweden,
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