| Modeling surface growth of Escherichia coli on agar plates. | |
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MedLine Citation:
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PMID: 16332768 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Surface growth of Escherichia coli cells on a membrane filter placed on a nutrient agar plate under various conditions was studied with a mathematical model. The surface growth of bacterial cells showed a sigmoidal curve with time on a semilogarithmic plot. To describe it, a new logistic model that we presented earlier (H. Fujikawa et al., Food Microbiol. 21:501-509, 2004) was modified. Growth curves at various constant temperatures (10 to 34 degrees C) were successfully described with the modified model (model III). Model III gave better predictions of the rate constant of growth and the lag period than a modified Gompertz model and the Baranyi model. Using the parameter values of model III at the constant temperatures, surface growth at various temperatures was successfully predicted. Surface growth curves at various initial cell numbers were also sigmoidal and converged to the same maximum cell numbers at the stationary phase. Surface growth curves at various nutrient levels were also sigmoidal. The maximum cell number and the rate of growth were lower as the nutrient level decreased. The surface growth curve was the same as that in a liquid, except for the large curvature at the deceleration period. These curves were also well described with model III. The pattern of increase in the ATP content of cells grown on a surface was sigmoidal, similar to that for cell growth. We discovered several characteristics of the surface growth of bacterial cells under various growth conditions and examined the applicability of our model to describe these growth curves. |
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Authors:
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Hiroshi Fujikawa; Satoshi Morozumi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 71 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-07 Completed Date: 2006-02-07 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 7920-6 Citation Subset: IM |
Affiliation:
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Tokyo Metropolitan Institute of Public Health Department of Microbiology, Shinjuku, Tokyo 169-0073, Japan. Hiroshi_1_Fujikawa@member.metro.tokyo.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Agar* Culture Media Escherichia coli / growth & development*, metabolism Kinetics Models, Biological Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 56-65-5/Adenosine Triphosphate; 9002-18-0/Agar |
| Comments/Corrections | |
Comment In:
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Appl Environ Microbiol. 2007 Apr;73(7):2402-3; author reply 2402-3
[PMID:
17403943
]
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Erratum In:
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Appl Environ Microbiol. 2007 Apr;73(7):2404 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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