Document Detail


Modeling shifts in microbial populations associated with health or disease.
MedLine Citation:
PMID:  16672517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stable microbial communities associated with health can be disrupted by altered environmental conditions. Periodontal diseases are associated with changes in the resident oral microflora. For example, as gingivitis develops, a key change in the microbial composition of dental plaque is the ascendancy of Actinomyces spp. and gram-negative rods at the expense of Streptococcus spp. We describe the use of an in vitro model to replicate this population shift, first with a dual-species model (Actinomyces naeslundii and Streptococcus sobrinus) and then using a microcosm model of dental plaque. The population shift was induced by environmental changes associated with gingivitis, first by the addition of artificial gingival crevicular fluid and then by a switch to a microaerophilic atmosphere. In addition to the observed population shifts, confocal laser scanning microscopy also revealed structural changes and differences in the distribution of viable and nonviable bacteria associated with the change in environmental conditions. This model provides an appropriate system for the further understanding of microbial population shifts associated with gingivitis and for the testing of, for example, antimicrobial agents.
Authors:
F Dalwai; D A Spratt; J Pratten
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-04     Completed Date:  2006-07-26     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3678-84     Citation Subset:  IM    
Affiliation:
Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. f.dalwai@eastman.ucl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Actinomyces / growth & development*
Bacteria / growth & development
Biofilms / growth & development*
Culture Media
Dental Plaque / microbiology*
Gingivitis / microbiology*
Humans
Image Processing, Computer-Assisted
Microscopy, Confocal
Models, Biological
Saliva
Streptococcus sobrinus / growth & development*
Chemical
Reg. No./Substance:
0/Culture Media
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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