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MODELING AND OPTIMIZATION OF GLUTAMIC ACID PRODUCTION USING MIXED CULTURE OF Corynebacterium glutamicum NCIM2168 AND Pseudomonas reptilivora NCIM2598.
MedLine Citation:
PMID:  23768112     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In this study, a hybrid system of response surface methodology followed by genetic algorithm has been adopted to optimize the production medium for L-glutamic acid fermentation with mixed cultures of Corynebacterium glutamicum and Pseudomonas reptilovora. The optimal combination of media components for maximal production of L-glutamic acid was found to be 49.99 g L(-1) of glucose, 10 g L(-1) of urea, 18.06% (v/v) of salt solution, and 4.99% (v/v) of inoculum size. The experimental glutamic acid yield at optimum condition was 19.69 g L(-1), which coincided well to the value predicted by the model (19.61 g L(-1)). Using this methodology, a nonlinear regression model was developed for the glutamic acid production. The model was validated statistically and the determination coefficient (R (2)) was found to be 0.99.
Authors:
Rajaram Shyam Kumar; Innasi Muthu Ganesh Moorthy; Rajoo Baskar
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Preparative biochemistry & biotechnology     Volume:  43     ISSN:  1532-2297     ISO Abbreviation:  Prep. Biochem. Biotechnol.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-06-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607037     Medline TA:  Prep Biochem Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  668-81     Citation Subset:  IM    
Affiliation:
a Department of Biotechnology , Centre for Research, Bioprocess and Downstream Processing Laboratory, Kamaraj College of Engineering and Technology , Virudhunagar , Tamilnadu , India.
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