Document Detail


Modeling and measurement of a whole-cell bioluminescent biosensor based on a single photon avalanche diode.
MedLine Citation:
PMID:  18774705     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Whole-cell biosensors are potential candidates for on-line and in situ environmental monitoring. In this work we present a new design of a whole-cell bioluminescence biosensor for water toxicity detection, based on genetically engineered Escherichia coli bacteria, carrying a recA::luxCDABE promoter-reporter fusion. Sensitive optical detection is achieved using a single photon avalanche photodiode (SPAD) working in the Geiger mode. The present work describes a simple mathematical model for the kinetic process of the bioluminescence based SOS toxin response of E. coli bacteria. We find that initially the bioluminescence signal depends on the time square and we show that the spectral intensity of the bioluminescence signal is inverse proportional to the frequency. We get excellent agreement between the theoretical model and the measured light signal. Furthermore, we present experimental results of the bioluminescent signal measurement using a SPAD and a photomultiplier, and demonstrate improvement of the measurement by applying a matched digital filter. Low intensity bioluminescence signals were measured after the whole-cell sensors were exposed to various toxicant concentrations (5, 15 and 20ppm).
Authors:
Ramiz Daniel; Ronen Almog; Amit Ron; Shimshon Belkin; Yosi Shacahm Diamand
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-25
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  24     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-20     Completed Date:  2009-02-12     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  888-93     Citation Subset:  IM    
Affiliation:
Department of Physical Electronic, Electrical Engineering Faculty, Tel Aviv University, Ramat Aviv, 69978 Tel-Aviv, Israel.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / administration & dosage
Biological Assay / instrumentation*,  methods
Biosensing Techniques / instrumentation*,  methods
Computer Simulation
Equipment Design
Equipment Failure Analysis
Escherichia coli / drug effects*,  physiology*
Luminescent Measurements / instrumentation*,  methods
Models, Biological*
Nalidixic Acid / administration & dosage*
Photons
Semiconductors*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 389-08-2/Nalidixic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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