Document Detail

Modeling lag phase of nonproteolytic Clostridium botulinum toxigenesis in cooked turkey and chicken breast as affected by temperature, sodium lactate, sodium chloride and spore inoculum.
MedLine Citation:
PMID:  8398625     Owner:  NLM     Status:  MEDLINE    
The length of the lag phase (LP) of toxigenesis in commercially cooked turkey meat stored under vacuum was determined as affected by 0, 1.2, 2 and 3% sodium lactate (L), 0, 1 and 2% NaCl (S), spore (pool of nonproteolytic B and E strains: B2, B17, B197, B706, E211, E250, E KA-2 and E Beluga) inoculum (I) of 10(-2) to 10(4), storage temperature (T) of 4, 8, 12, 16, 20 and 30 degrees C and their interactions. The time from inoculation to the detection of first toxic sample was defined as LP. Using regression analysis the following model predictive of LP of C. botulinum toxigenesis in the cooked turkey breast was derived: Log(1/LP) = -2.2877 -0.1235(S) -0.2174(L) +0.4391(square root of T) +0.0204(square root of T) (I). The model explained 94.5% of the variation in results, in which square root of T was the most influential factor (65%), followed by L (21.2%), interaction of I and square root of T (4.9%) and S (3.4%). The model predicted LPs longer than those observed in 28.3% of the comparisons, but only in 1% of the comparisons when the lower limit of the 90% confidence interval of LP was used. Similar trends on the effect of L on C. botulinum were observed in an inoculated chicken meat study. This study demonstrated quantitatively that increasing L and S concentrations and lowering of T had a beneficial effect on delaying toxigenesis.
J Meng; C A Genigeorgis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of food microbiology     Volume:  19     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-10-28     Completed Date:  1993-10-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  109-22     Citation Subset:  IM    
Department of Epidemiology & Preventive Medicine, School of Veterinary Medicine, University of California, Davis.
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MeSH Terms
Analysis of Variance
Botulinum Toxins / biosynthesis*
Botulism / etiology
Clostridium botulinum / drug effects,  metabolism*
Dose-Response Relationship, Drug
Food Handling / methods*
Food Microbiology*
Lactates / pharmacology
Lactic Acid
Meat / microbiology*
Models, Biological
Regression Analysis
Sodium Chloride / pharmacology
Spores, Bacterial
Time Factors
Reg. No./Substance:
0/Botulinum Toxins; 0/Lactates; 50-21-5/Lactic Acid; 7647-14-5/Sodium Chloride

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