Document Detail

Modeling the interactions of particulates with epithelial lining fluid antioxidants.
MedLine Citation:
PMID:  10516212     Owner:  NLM     Status:  MEDLINE    
Oxidative stress may be a fundamental mode of injury associated with inspired particles. To examine this, we determined the ability of three carbon black particles (CBPs; M120, M880, and R250) and two forms of silicon dioxide, amorphous (Cabosil) and crystalline (DQ12) quartz, to deplete epithelium lining fluid antioxidant defenses. Single and composite antioxidant solutions of uric acid, ascorbic acid (AA), and reduced glutathione (GSH) were examined in the presence of particle concentrations of 150 microgram/ml. Uric acid was not depleted by any particle considered. AA was depleted in a near-linear fashion with time by the three different CBPs; however, AA depletion rates varied markedly with CBP type and decreased in the presence of metal chelators. An initially high GSH depletion rate was noted with all CBPs, and this was always accompanied by the appearance of oxidized glutathione. Exposure to Cabosil or DQ12 did not result in the loss of GSH. Together, these data demonstrate that particle type, size, and surface area are all important factors when considering particle-antioxidant interactions in the airways.
H Zielinski; I S Mudway; K A Bérubé; S Murphy; R Richards; F J Kelly
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-22     Completed Date:  1999-11-22     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  L719-26     Citation Subset:  IM    
Institute of Animal Reproduction, Polish Academy of Sciences, Olsztyn 10-747, Poland.
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MeSH Terms
Air Pollutants / pharmacology*
Antioxidants / metabolism*
Ascorbic Acid / metabolism
Body Fluids / metabolism*
Carbon / pharmacology
Drug Interactions
Epithelium / metabolism
Glutathione / metabolism
Glutathione Disulfide / biosynthesis
Lung / drug effects*,  metabolism*
Silicon Dioxide / pharmacology
Uric Acid / metabolism
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Air Pollutants; 0/Antioxidants; 0/Solutions; 27025-41-8/Glutathione Disulfide; 50-81-7/Ascorbic Acid; 69-93-2/Uric Acid; 70-18-8/Glutathione; 7440-44-0/Carbon; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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