Document Detail

Modeling of high cell density fed batch cultivation.
MedLine Citation:
PMID:  8011358     Owner:  NLM     Status:  MEDLINE    
Escherichia coli was grown in carbon- and energy source-limited fed batch cultures to study the effect of osmotic stress and different feed rates on the growth kinetics. An unstructured model based on the linear equation for substrate consumption provided an adequate description of the bacterial growth during the first phase of biomass production (20 h), except for cultures exposed to osmotic stress by the addition of 0.5 M NaCl. The addition of salt to the culture media had a large effect on the energetics, that could not simply be described in terms of an increased maintenance requirement. In the later phase of growth, an extensive decline in viability for all cultures was observed. Coincidentally, the specific sugar uptake rate approached a lower limit. It is concluded that the total obtainable biomass in a fed batch culture is strongly affected by the magnitudes of the substrate feed rate and the ionic strength of the culture medium.
L Andersson; L Strandberg; L Häggström; S O Enfors
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology reviews     Volume:  14     ISSN:  0168-6445     ISO Abbreviation:  FEMS Microbiol. Rev.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-07-28     Completed Date:  1994-07-28     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8902526     Medline TA:  FEMS Microbiol Rev     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  39-44     Citation Subset:  IM    
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
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MeSH Terms
Culture Media
Escherichia coli / growth & development*,  metabolism
Glucose / metabolism
Models, Biological*
Osmolar Concentration
Oxygen Consumption
Reg. No./Substance:
0/Culture Media; 50-99-7/Glucose

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