Document Detail


Modeling electroporation in a single cell. II. Effects Of ionic concentrations.
MedLine Citation:
PMID:  10465737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study expands a previously developed model of a single cell electroporated by an external electric field by explicitly accounting for the ionic composition of the electroporation current. The previous model with non-specific electroporation current predicts that both the transmembrane potential V(m) and the pore density N are symmetric about the equator, with the same values at either end of the cell. The new, ion-specific case predicts that V(m) is symmetric and almost identical to the profile from the non-specific case, but N has a profound asymmetry with the pore density at the hyperpolarized end of the cell twice the value at the depolarized end. These modeling results agree with the experimentally observed preferential uptake of marker molecules at the hyperpolarized end of the cell as reported in the literature. This study also investigates the changes in intracellular ionic concentrations induced around an electroporated single cell. For all ion species, the concentrations near the membrane vary significantly, which may explain the electrical disturbances observed experimentally after large electric shocks are delivered to excitable cells and tissues.
Authors:
K A DeBruin; W Krassowska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  77     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-08     Completed Date:  1999-10-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1225-33     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering and Center for Emerging Cardiovascular Technologies, Duke University, Durham, North Carolina 27708-0281 USA. kad3@eel-mail.mc.duke.edu
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MeSH Terms
Descriptor/Qualifier:
Cell Physiological Phenomena*
Electroporation* / methods
Ions
Mathematics
Models, Biological*
Osmolar Concentration
Grant Support
ID/Acronym/Agency:
HL54071/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Ions
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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