| Modeling of effects of nutrient gradients on cell proliferation in microfluidic bioreactor. | |
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MedLine Citation:
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PMID: 17935347 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Precise control of the microenvironment is highly desirable in cell culture to study the cell biology. Microfluidic based bioreactors provide a promising method for the spatial and temporal control of cell growth and stimuli. A three-dimensional nutrient transport model, incorporating the monolayer cell growth model, has been developed to investigate the influence of gradients of oxygen and epidermal growth factor (EGF) on the cell culture in the continuous-flow microchannel bioreactor. Our results demonstrate that applying inlet concentration gradients of oxygen and EGF can induce variations of cell density in the y-direction. It is further found that compared to the oxygen gradients the EGF concentration gradients are more efficient in regulating the cell growth. |
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Authors:
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Guoqing Hu; Vito Quaranta; Dongqing Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-13 |
Journal Detail:
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Title: Biotechnology progress Volume: 23 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2007 Nov-Dec |
Date Detail:
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Created Date: 2007-12-07 Completed Date: 2008-03-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 1347-54 Citation Subset: IM |
Affiliation:
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Department of Mechanical Engineering, Vanderbilt University VU Station B 351592, 2301 Vanderbilt Place, Nashville, Tennessee 37235-1592, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bioreactors* Cell Count Cell Proliferation / drug effects* Cells, Cultured Epidermal Growth Factor / pharmacology Humans Microfluidic Analytical Techniques* Models, Theoretical Oxygen / pharmacology |
| Chemical | |
Reg. No./Substance:
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62229-50-9/Epidermal Growth Factor; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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