Document Detail


Modeling amino acid metabolism in mammalian cells-toward the development of a model library.
MedLine Citation:
PMID:  17949105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amino acids are necessary to mammalian cell cultures both for protein synthesis and as an energy source. In this study, we present an unstructured mathematical model describing (i) cell growth and death kinetics and (ii) metabolism of glucose and 19 amino acids for HEK-293 and CHO IFN-gamma cell cultures. The proposed mathematical framework is in good agreement with experimental data for both cell lines. It accommodates the inclusion of expressions for other cellular activities, such as the production of recombinant viral vectors or proteins, and can be used as the basis for the development of a model library for mammalian cell cultures.
Authors:
Cleo Kontoravdi; Danny Wong; Carolyn Lam; Yih Yean Lee; Miranda G S Yap; Efstratios N Pistikopoulos; Athanasios Mantalaris
Related Documents :
22285005 - Bacteriuria and antibiotic resistance in catheter urine specimens following radical pro...
25372565 - Disordered eating and cultural distinctions: exploring prevalence and predictors among ...
8540335 - Expression of human cathepsin e in methylotrophic yeast, pichia pastoris.
14657355 - Circadian gene expression in mammalian fibroblasts revealed by real-time luminescence r...
22222535 - Isolation of cardiac myocytes and fibroblasts from neonatal rat pups.
9706145 - Butyrate inhibits colon carcinoma cell growth through two distinct pathways.
Publication Detail:
Type:  Journal Article     Date:  2007-10-20
Journal Detail:
Title:  Biotechnology progress     Volume:  23     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2007-12-07     Completed Date:  2008-03-04     Revised Date:  2008-03-13    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1261-9     Citation Subset:  IM    
Affiliation:
Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
CHO Cells
Cricetinae
Cricetulus
Databases, Factual*
Glucose / metabolism
Models, Theoretical
Chemical
Reg. No./Substance:
0/Amino Acids; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Thermal behavior of Bombyx mori silk: evolution of crystalline parameters, molecular structure, and ...
Next Document:  Adapted ultra scale-down approach for predicting the centrifugal separation behavior of high cell de...