Document Detail

Modeling of DR CALUX bioassay response to screen PCDDs, PCDFs, and dioxin-like PCBs in farm milk from dairy herds.
MedLine Citation:
PMID:  18295947     Owner:  NLM     Status:  MEDLINE    
A recent issue in the EU legislation is the evaluation of the toxicologically-equivalent contribution of dioxin-like polychlorobiphenyls (DL-PCBs) in addition to that coming from polychlorodibenzodioxins (PCDDs) and polychlorodibenzofurans (PCDFs) as contaminants in foods for a total of 29 congeners. This fact is determining the need to revise analytical criteria both for confirmatory and screening analysis. In this work, a modeling was developed to check the reliability of the outcomes of the DR CALUX bioassay when applied to farm milk samples characterized by large differences in congener patterns. To reproduce some field conditions where DL-PCB contributions up to 90% of total WHO-TEQs (HRGC-HRMS assessment) were recorded in dairy products, goat milk samples from a common bulk were fortified at different TEQ levels with mixtures containing either PCDDs and PCDFs or non-ortho substituted DL-PCBs. Fortification ranged approximately 4.5-15 pgWHO-TEQ/g fat. Based on the results, DR CALUX relative potency value (REP) of DL-PCB 126 was estimated 0.061 against the canonical WHO-TEF of 0.1. The value of 0.061 together with the other DR CALUX REPs from the literature for the remaining 28 congeners were used to model DR CALUX response (C-TEQs) in milk samples with different congener patterns. The theoretical underestimation of DR CALUX data could be mitigated by correcting the latter with the linear correlation experimentally obtained between C-TEQs and the WHO-TEQs. Under these conditions, the use as calibrants of reference samples with different analytical patterns could help those laboratories involved in a high throughput routine to set the most appropriate decision limits to optimize screening output.
Igor Fochi; Gianfranco Brambilla; Stefania P De Filippis; Silvia De Luca; Gianfranco Diletti; Annarita Fulgenzi; Pasquale Gallo; Nicola Iacovella; Giampiero Scortichini; Luigi Serpe; Floriana Vinci; Alessandro di Domenico
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-26
Journal Detail:
Title:  Regulatory toxicology and pharmacology : RTP     Volume:  50     ISSN:  0273-2300     ISO Abbreviation:  Regul. Toxicol. Pharmacol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-21     Completed Date:  2008-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8214983     Medline TA:  Regul Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  366-75     Citation Subset:  IM    
Istituto Superiore di Sanità, Toxicological Chemistry Unit, Viale Regina Elena, 299 I-00161 Rome, Italy.
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MeSH Terms
Benzofurans / analysis,  toxicity*
Biological Assay / methods*
Chromatography, High Pressure Liquid
Data Interpretation, Statistical
Environmental Pollutants / analysis,  toxicity*
Linear Models
Milk / chemistry*
Models, Statistical
Polychlorinated Biphenyls / analysis,  toxicity*
Polymers / analysis,  toxicity*
Reference Standards
Reproducibility of Results
Tetrachlorodibenzodioxin / analogs & derivatives*,  analysis,  toxicity
Reg. No./Substance:
0/Benzofurans; 0/Environmental Pollutants; 0/Polychlorinated Biphenyls; 0/Polymers; 0/polychlorodibenzo-4-dioxin; 0/polychlorodibenzofuran; 1746-01-6/Tetrachlorodibenzodioxin

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