Document Detail


Model for nonequilibrium binding and affinity chromatography: characterization of 8-hydroxyquinoline immobilized on controlled pore glass using a flow injection system with a packed microcolumn
MedLine Citation:
PMID:  10959984     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This paper discusses the use of pulse techniques for analysis of zonal elution data for the determination of mass-transfer and axial dispersion constants for porous support materials with adsorption to the surface or to a surface-bonded phase. As an example, this paper considers the case of controlled pore glass (CPG) with a bonded phase that is used with microcolumns and a flow injection analysis system. For the CPG, axial dispersion in the form of eddy mixing can be described by l = 0.203, and the overall mass-transfer term, K(OL) = 3.9 x 10(-6) cm/s. Additionally, an affinity chromatography model was adapted to effectively describe systems employing CPG as the support material through modification of equations describing typical affinity chromatography systems and by inclusion of an axial dispersion term in the calculation of N. This model was used to predict breakthrough curves for cadmium adsorption by 8-hydroxyquinolinol immobilized on CPG packed in microcolumns. In general, the information from the model can be used to extract equilibrium-based constants (binding strengths and site capacities) from a nonequilibrium flow system. The data and model can also be employed in determining the performance for scaled-up extraction systems. The modified model is available in EXCEL spreadsheet format as Supporting Information.
Authors:
Howard; Holcombe
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Analytical chemistry     Volume:  72     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-08-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  Eng     Pagination:  3927-33     Citation Subset:  -    
Affiliation:
Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, USA.
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