Document Detail


Model-based automated detection of mammalian cell colonies.
MedLine Citation:
PMID:  11720364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Manually counting cell colonies, especially those that originate from fibroblast cell lines, is a time-consuming, eye-straining and tedious task in which consistency of counting is difficult to maintain. In this paper we present a novel model-based image segmentation method, which employs prior knowledge about the shape of a colony with the aim to automatically detect isolated, touching and overlapping cell colonies of various sizes and intensities. First, a set of hypothetical model instances is generated by using a robust statistical approach to estimate the model parameters and a novel confidence measure to quantify the difference between a model instance and the underlying image. Second, the model instances matching the individual colonies in the image are selected from the set by a minimum description length principle. The procedure was applied to images of Chinese hamster lung fibroblast cell line DC3F, which forms poorly defined or 'fuzzy' colonies. The correlation with manual counting was determined and the cell survival curves obtained by automated and manual counting were compared. The results obtained show that the proposed automatic procedure was capable to correctly identify 91% of cell colonies typical of mammalian cell lines.
Authors:
R Bernard; M Kanduser; F Pernus
Related Documents :
7444714 - Techniques for purifying l-cell karyoplasts with minimal amounts of cytoplasm.
7664854 - Some technical factors influencing the induction of sputum for cell analysis.
3973394 - A simple technique for evaluation of methods of cell separation.
6378874 - Comparison of semiautomated method with official optical somatic cell counting method i...
17146594 - Expression of cyp2e1 in human nasopharynx and its metabolic effect in vitro.
23045034 - Analytical cytology: applications to neurotoxicology.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Physics in medicine and biology     Volume:  46     ISSN:  0031-9155     ISO Abbreviation:  Phys Med Biol     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-26     Completed Date:  2002-04-29     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0401220     Medline TA:  Phys Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3061-72     Citation Subset:  IM    
Affiliation:
Faculty of Electrical Engineering, University of Ljubljana, Slovenia. rok.bernard@fe.uni-lj.si
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Automation
Cell Count
Cell Line
Cell Separation
Cell Survival
Cells, Cultured
Cricetinae
Cytological Techniques / methods*
Electroporation
Fibroblasts / cytology
Models, Theoretical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A model of the electrical volume conductor in the region of the eye in the ELF range.
Next Document:  Comparison of conversion factors for x-ray beams from a Philips SL15 and the NPL linear accelerator.