| A model for cleavage plane determination in early amphibian and fish embryos. | |
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MedLine Citation:
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PMID: 21055946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Current models for cleavage plane determination propose that metaphase spindles are positioned and oriented by interactions of their astral microtubules with the cellular cortex, followed by cleavage in the plane of the metaphase plate [1, 2]. We show that in early frog and fish embryos, where cells are unusually large, astral microtubules in metaphase are too short to position and orient the spindle. Rather, the preceding interphase aster centers and orients a pair of centrosomes prior to nuclear envelope breakdown, and the spindle assembles between these prepositioned centrosomes. Interphase asters center and orient centrosomes with dynein-mediated pulling forces. These forces act before astral microtubules contact the cortex; thus, dynein must pull from sites in the cytoplasm, not the cell cortex as is usually proposed for smaller cells. Aster shape is determined by interactions of the expanding periphery with the cell cortex or with an interaction zone that forms between sister-asters in telophase. We propose a model to explain cleavage plane geometry in which the length of astral microtubules is limited by interaction with these boundaries, causing length asymmetries. Dynein anchored in the cytoplasm then generates length-dependent pulling forces, which move and orient centrosomes. |
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Authors:
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Martin Wühr; Edwin S Tan; Sandra K Parker; H William Detrich; Timothy J Mitchison |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-11-04 |
Journal Detail:
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Title: Current biology : CB Volume: 20 ISSN: 1879-0445 ISO Abbreviation: Curr. Biol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-04-18 Revised Date: 2012-02-22 |
Medline Journal Info:
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Nlm Unique ID: 9107782 Medline TA: Curr Biol Country: England |
Other Details:
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Languages: eng Pagination: 2040-5 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. martin.wuehr@gmx.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division* Centrosome / ultrastructure Embryo, Nonmammalian / cytology*, ultrastructure Embryonic Development Female Fertilization Male Metaphase Microtubules / ultrastructure Mitotic Spindle Apparatus / metabolism, ultrastructure Models, Biological* Spermatozoa / ultrastructure Xenopus laevis Zebrafish / embryology |
| Grant Support | |
ID/Acronym/Agency:
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GM39565/GM/NIGMS NIH HHS; R01 GM039565-23/GM/NIGMS NIH HHS; R01 GM039565-25/GM/NIGMS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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