| Mode of regulation and the insulation of bacterial gene expression. | |
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MedLine Citation:
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PMID: 22633488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A gene can be said to be insulated from environmental variations if its expression level depends only on its cognate inducers, and not on variations in conditions. We tested the insulation of the lac promoter of E. coli and of synthetic constructs in which the transcription factor CRP acts as either an activator or a repressor, by measuring their input function-their expression as a function of inducers-in different growth conditions. We find that the promoter activities show sizable variation across conditions of 10%-100% (SD/mean). When the promoter is bound to its cognate regulator(s), variation across conditions is smaller than when it is unbound. Thus, mode of regulation affects insulation: activators seem to show better insulation at high expression levels, and repressors at low expression levels. This may explain the Savageau demand rule, in which E. coli genes needed often in the natural environment tend to be regulated by activators, and rarely needed genes by repressors. The present approach can be used to study insulation in other genes and organisms. |
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Authors:
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Vered Sasson; Irit Shachrai; Anat Bren; Erez Dekel; Uri Alon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular cell Volume: 46 ISSN: 1097-4164 ISO Abbreviation: Mol. Cell Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-28 Completed Date: 2012-07-27 Revised Date: 2012-08-03 |
Medline Journal Info:
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Nlm Unique ID: 9802571 Medline TA: Mol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 399-407 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cyclic AMP
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metabolism Cyclic AMP Receptor Protein / metabolism Escherichia coli / genetics*, metabolism Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial* Genes, Bacterial Genes, Reporter Lac Operon Mathematical Concepts Models, Genetic Promoter Regions, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Cyclic AMP Receptor Protein; 0/Escherichia coli Proteins; 0/crp protein, E coli; 60-92-4/Cyclic AMP |
| Comments/Corrections | |
Comment In:
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Mol Cell. 2012 Jun 8;46(5):551-2
[PMID:
22681881
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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