| Mode of growth hormone administration influences triacylglycerol synthesis and assembly of apolipoprotein B-containing lipoproteins in cultured rat hepatocytes. | |
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MedLine Citation:
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PMID: 9026526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypophysectomized female rats were treated for 1 week with thyroxine (10 micrograms/kg.day), cortisol (400 micrograms/kg.day), and bovine GH (1 mg/kg.day) either as two daily subcutaneous injections (GH x 2) or as a continuous subcutaneous infusion (GHc) in order to mimic the male and female specific GH secretory patterns, respectively. Hepatocytes were then isolated and kept in short-term cultures. Hypophysectomy decreased the synthesis of triacylglycerol. Treatment with GH x 2 had no or small effects, while GHc normalized the effect of hypophysectomy. ApoB-100 VLDL was assembled before apoB-48 VLDL. ApoB-48 was first assembled as an HDL particle (apoB-48 "HDL"). Hypophysectomy decreased the proportion of intracellular apoB-48 that was recovered as VLDL. Moreover, the proportion of apoB-48 of total apoB in VLDL decreased. Only GHc fully restored the effect of hypophysectomy by inducing an 4-fold increase in the assembly of apoB-48 VLDL, while treatment with GH x 2 gave rise to a 1.8-fold increase. Hypophysectomy resulted in a decrease in the proportion of apoB-48 that was secreted as VLDL and a decrease in the proportion of apoB-48 of total apoB in VLDL. Only treatment with GHc fully restored the secretion of apoB-48 VLDL by inducing an almost 4-fold increase in the secretion of apoB-48 VLDL, while the corresponding value for treatment with GH x 2 was 1.7. However, GH x 2 increased the proportion of the secreted apoB-48 that was recovered in VLDL to the levels found in normal rats and in rats treated with GHc, but this finding was due to a failure of GH x 2 treatment to increase the secretion of apoB-48 "HDL". In summary, a continuous infusion of GH to hypophysectomized rats, mimicking the female secretion of GH, normalized the triacylglycerol synthesis and secretion as well as apoB-48 VLDL assembly and secretion to those levels observed in hepatocyte cultures from intact female rats. |
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Authors:
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A Sjöberg; J Oscarsson; J Borén; S Edén; S O Olofsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of lipid research Volume: 37 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1997-02-20 Completed Date: 1997-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 275-89 Citation Subset: IM |
Affiliation:
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Department of Medical Biochemistry, Góteborg University, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoprotein B-100 Apolipoprotein B-48 Apolipoproteins B / metabolism* Cattle Cells, Cultured Female Glycerol / metabolism Growth Hormone / administration & dosage*, pharmacology Hypophysectomy Kinetics Lipoproteins / biosynthesis* Liver / drug effects, metabolism* Oleic Acid / metabolism Rats Rats, Sprague-Dawley Triglycerides / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein B-100; 0/Apolipoprotein B-48; 0/Apolipoproteins B; 0/Lipoproteins; 0/Triglycerides; 112-80-1/Oleic Acid; 56-81-5/Glycerol; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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