Document Detail

Mode of action and safety of lactosporin, a novel antimicrobial protein produced by Bacillus coagulans ATCC 7050.
MedLine Citation:
PMID:  22737982     Owner:  NLM     Status:  MEDLINE    
AIMS: To determine the mechanism of action of antimicrobial protein, lactosporin, against Gardnerella vaginalis and to evaluate its safety in vitro.
METHODS AND RESULTS: Bacillus coagulans ATCC 7050 was grown at 37°C for 18 h. The cell-free supernatant was concentrated 10-fold and screened for antimicrobial activity against indicator strain Micrococcus luteus. The mode of action of lactosporin was determined by measuring the potassium release and monitoring the changes in transmembrane potential (Δψ) and transmembrane pH (ΔpH) of the sensitive cells. Lactosporin caused the efflux of potassium ions from M. luteus cells and dissipation of ΔpH in G. vaginalis, while it had no effect on the Δψ. The safety of lactosporin was evaluated by using EpiVaginal(™) ectocervical (VEC-100) tissue model. Over 80% of the cells in the vaginal tissue remained viable after exposure to lactosporin for 24 h.
CONCLUSIONS: Lactosporin potentially exerts its antimicrobial activity by selective dissipation of ΔpH and/or by causing leakage of ions from the sensitive cells. Safety studies suggest that lactosporin is a noncytotoxic antimicrobial for vaginal application.
SIGNIFICANCE AND IMPACT OF THE STUDY: This study revealed that lactosporin is an effective and safe antimicrobial preparation with potential application for the control of bacterial vaginosis.
S Riazi; S E Dover; M L Chikindas
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-24
Journal Detail:
Title:  Journal of applied microbiology     Volume:  113     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-15     Completed Date:  2013-05-29     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  714-22     Citation Subset:  IM    
Copyright Information:
No claim to US Government works Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA.
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MeSH Terms
Anti-Bacterial Agents / metabolism,  pharmacology*
Bacillus / metabolism*
Bacteriocins / metabolism,  pharmacology*
Gardnerella vaginalis / drug effects*,  growth & development
Hydrogen-Ion Concentration
Membrane Potentials / drug effects
Micrococcus luteus / drug effects
Potassium / analysis
Proton-Motive Force
Vagina / cytology,  drug effects,  microbiology
Vaginosis, Bacterial / drug therapy
Grant Support
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacteriocins; 7440-09-7/Potassium

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