| Mode of action of amphotericin B on chick embryo fibroblasts and on mouse Ehrlich tumour cells: a cytological and cytochemical analysis. | |
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MedLine Citation:
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PMID: 1158980 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chick embryo fibroblasts cultivated in vitro and Ehrlich ascites tumor cells (in vivo or in vitro) have been treated with amphotericin B. Cell multiplication is strongly inhibited. Large clear zones appear in the fibroblast nucleoi (phase-contrast and electron-microscope observations). Many treated fibroblasts and tumour cells have a high DNA content (pre-mitotic or polyploid level; measurements by cytophotometry). However, the RNA content (cytophotometry) and the total protein content (cytophotometry and micro-interferometry) are relatively low in the tumour cells. As shown by autoradiography, DNA synthesis is active but RNA synthesis and, in some cases, protein synthesis are inhibited. Due to this unbalanced growth, the cells cannot divide. |
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Authors:
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F De Paermentier; R Bassleer; A Lepoint; C Desaive; G Goessens; M P Lhoest-Gauthier |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cell science Volume: 18 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 1975 Aug |
Date Detail:
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Created Date: 1975-11-22 Completed Date: 1975-11-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 441-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amphotericin B
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pharmacology* Animals Carcinoma, Ehrlich Tumor* / metabolism, pathology Cell Division / drug effects Cell Nucleus / ultrastructure Chick Embryo Culture Techniques Cytoplasm / ultrastructure DNA / biosynthesis DNA, Neoplasm / biosynthesis Fibroblasts / drug effects*, metabolism, ultrastructure Lipids Mice Mitosis / drug effects* Mitotic Index Neoplasm Proteins / biosynthesis Protein Biosynthesis RNA / biosynthesis RNA, Neoplasm / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Lipids; 0/Neoplasm Proteins; 0/RNA, Neoplasm; 1397-89-3/Amphotericin B; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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