Document Detail

Mode of action of amphotericin B on chick embryo fibroblasts and on mouse Ehrlich tumour cells: a cytological and cytochemical analysis.
MedLine Citation:
PMID:  1158980     Owner:  NLM     Status:  MEDLINE    
Chick embryo fibroblasts cultivated in vitro and Ehrlich ascites tumor cells (in vivo or in vitro) have been treated with amphotericin B. Cell multiplication is strongly inhibited. Large clear zones appear in the fibroblast nucleoi (phase-contrast and electron-microscope observations). Many treated fibroblasts and tumour cells have a high DNA content (pre-mitotic or polyploid level; measurements by cytophotometry). However, the RNA content (cytophotometry) and the total protein content (cytophotometry and micro-interferometry) are relatively low in the tumour cells. As shown by autoradiography, DNA synthesis is active but RNA synthesis and, in some cases, protein synthesis are inhibited. Due to this unbalanced growth, the cells cannot divide.
F De Paermentier; R Bassleer; A Lepoint; C Desaive; G Goessens; M P Lhoest-Gauthier
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cell science     Volume:  18     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1975 Aug 
Date Detail:
Created Date:  1975-11-22     Completed Date:  1975-11-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  441-51     Citation Subset:  IM    
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MeSH Terms
Amphotericin B / pharmacology*
Carcinoma, Ehrlich Tumor* / metabolism,  pathology
Cell Division / drug effects
Cell Nucleus / ultrastructure
Chick Embryo
Culture Techniques
Cytoplasm / ultrastructure
DNA / biosynthesis
DNA, Neoplasm / biosynthesis
Fibroblasts / drug effects*,  metabolism,  ultrastructure
Mitosis / drug effects*
Mitotic Index
Neoplasm Proteins / biosynthesis
Protein Biosynthesis
RNA / biosynthesis
RNA, Neoplasm / biosynthesis
Reg. No./Substance:
0/DNA, Neoplasm; 0/Lipids; 0/Neoplasm Proteins; 0/RNA, Neoplasm; 1397-89-3/Amphotericin B; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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