| Mobilization of different arachidonate pools and their roles in the generation of leukotrienes and free arachidonic acid during immunologic activation of mast cells. | |
| | |
MedLine Citation:
|
PMID: 8419489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Immunologic activation of mast cells leads to the mobilization of arachidonic acid (AA) from membrane phospholipids and the subsequent conversion of this AA to bioactive products. The objective of our study was to determine if segregated pools of AA-containing phospholipids within mast cells serve as independent sources of AA. Initial studies indicated that the appearance of free AA occurred rapidly (maximal level formed within 1 min) within supernatant fluids of Ag-stimulated mast cells and was kinetically different from the formation of leukotriene (LT) B4 or LTC4. To examine whether free AA and leukotrienes were mobilized from different sources, AA-containing phospholipids of mast cells were labeled with [14C] and [3H] AA such that all major subclasses (1-acyl-1-alkyl-1-alk-1'-enyl) of phospholipids contained different ratios of [3H] to [14C] (sp. act. ratios (SAR)). Mast cells were then stimulated with Ag and the SAR of cellular AA, extracellular AA and extracellular LTC4, LTB4, 6-trans LTB4, were determined. The SAR were uniform in all LT and mimicked the ratio found in cellular AA. By contrast, the SAR of AA released into supernatant fluids was twofold lower than that of LT. This indicated that AA released as free fatty acid clearly was derived from a different lipid pool than AA that formed LT. Although it was apparent that the pools which gave rise to AA and LT were different, defining phospholipid(s) that constitute these distinct pools proved more difficult. The SAR of LT suggested that their cellular precursor AA could have been derived from several phospholipid subclasses; however, the SAR in phosphatidylcholine and phosphatidylinositol most closely matched the LT. The SAR of AA in supernatant fluids implied that it was derived, in part, from phosphatidylethanolamine subclasses. Taken together, these data suggest that there are at least two different pools of AA that are mobilized in mast cells during Ag activation. |
| | |
Authors:
|
A N Fonteh; F H Chilton |
Related Documents
:
|
1326069 - Inhibition of cholesterol synthesis by cyclopropylamine derivatives of squalene in huma... 8893159 - Vldl and ldl metabolism in human and rat mesangial cells. 8389299 - Regulation of low-density-lipoprotein receptors in the human hepatoma cell line hep g2.... 1332629 - Xanthomatized atypical t cells in a patient with mycosis fungoides and hyperlipidemia. 20979229 - Invariant natural killer t cells: linking inflammation and neovascularization in human ... 3355529 - Alpha-adrenergic suppression of very-low-density-lipoprotein triacylglycerol secretion ... 11767279 - An adenoviral system for tetracycline-regulated tgf-beta expression mediates a reversib... 17393359 - Differential expression patterns of irf3 and irf7 in pediatric lymphoid disorders. 9168799 - Role of heme-hemopexin in human t-lymphocyte proliferation. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 150 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1993 Jan |
Date Detail:
|
Created Date: 1993-02-05 Completed Date: 1993-02-05 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 563-70 Citation Subset: AIM; IM |
Affiliation:
|
Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27157-1054. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens / immunology* Arachidonic Acid / metabolism* Female Leukotrienes / biosynthesis* Mast Cells / immunology* Mice Mice, Inbred CBA Phospholipids / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
AI-24985/AI/NIAID NIH HHS; AI-26771/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens; 0/Leukotrienes; 0/Phospholipids; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A role for gamma delta + T cells during experimental infection of mice with Leishmania major.
Next Document: Activation of the C4 and C2 components of complement by a proteinase in serum bactericidal factor, R...