Document Detail


Mobilization of CD34/CXCR4+, CD34/CD117+, c-met+ stem cells, and mononuclear cells expressing early cardiac, muscle, and endothelial markers into peripheral blood in patients with acute myocardial infarction.
MedLine Citation:
PMID:  15533859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Adult stem cells can contribute to myocardial regeneration after ischemic injury. Bone marrow and skeletal muscles contain a population of CXCR4+ cells expressing genes specific for muscle progenitor cells that can be mobilized into the peripheral blood. The aims of the study were (1) to confirm the presence of early tissue-committed cells expressing cardiac, muscle, and endothelial markers in populations of mononuclear cells in peripheral blood and (2) to assess the dynamics and magnitude of the mobilization of CD34+, CD117+, CXCR4+, c-met+, CD34/CD117+, and CD34/CXCR4+ stem cells into peripheral blood in relation to inflammatory and hematopoietic cytokines in patients with ST-segment-elevation acute myocardial infarction (STEMI). METHODS AND RESULTS: Fifty-six patients with STEMI (<12 hours), 39 with stable angina, and 20 healthy control subjects were enrolled. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used for detection of tissue-specific markers. The number of the cells was assessed by use of a flow cytometer on admission, after 24 hours, and after 7 days. RT-PCR revealed increased expression of mRNA (up to 3.5-fold increase) for specific cardiac (GATA4, MEF2C, Nkx2.5/Csx), muscle (Myf5, Myogenin, MyoD), and endothelial (VE-cadherin, von Willebrand factor) markers in peripheral blood mononuclear cells. The number of CD34/CXCR4+ and CD34/CD117+ and c-met+ stem cells in peripheral blood was significantly higher in STEMI patients than in stable angina and healthy subjects, peaking on admission, without further significant increase after 24 hours and 7 days. CONCLUSIONS: The study demonstrates in the setting of STEMI a marked mobilization of mononuclear cells expressing specific cardiac, muscle, and endothelial markers as well as CD34/CXCR4+ and CD34/CD117+ and c-met+ stem cells and shows that stromal cell-derived factor-1 is an important factor influencing the mobilization.
Authors:
Wojciech Wojakowski; Michał Tendera; Anna Michałowska; Marcin Majka; Magdalena Kucia; Katarzyna Maślankiewicz; Rafał Wyderka; Andrzej Ochała; Mariusz Z Ratajczak
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-08
Journal Detail:
Title:  Circulation     Volume:  110     ISSN:  1524-4539     ISO Abbreviation:  Circulation     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-16     Completed Date:  2005-06-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3213-20     Citation Subset:  AIM; IM    
Affiliation:
Third Division of Cardiology, Silesian School of Medicine, 45-47 Ziołowa St, 40-635 Katowice, Poland. wojwoj@mp.pl
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MeSH Terms
Descriptor/Qualifier:
Adult
Angina Pectoris / blood,  physiopathology
Antigens, CD34 / analysis
Antigens, Differentiation / analysis*
Blood Cell Count
Chemokine CXCL12
Chemokines, CXC / blood,  physiology
Cytokines / blood*
Endothelium / cytology
Female
Flow Cytometry
Follow-Up Studies
Granulocyte Colony-Stimulating Factor / blood
Hepatocyte Growth Factor / blood
Humans
Inflammation / blood
Interleukin-6 / blood
Leukocytes, Mononuclear / chemistry,  physiology*
Male
Middle Aged
Multipotent Stem Cells / chemistry,  physiology*
Muscles / cytology
Myocardial Infarction / blood*,  physiopathology
Myocardium / cytology
Proto-Oncogene Proteins c-kit / analysis
Proto-Oncogene Proteins c-met / analysis
Receptors, CXCR4 / analysis
Reverse Transcriptase Polymerase Chain Reaction
Vascular Endothelial Growth Factor A / blood
Chemical
Reg. No./Substance:
0/Antigens, CD34; 0/Antigens, Differentiation; 0/CXCL12 protein, human; 0/Chemokine CXCL12; 0/Chemokines, CXC; 0/Cytokines; 0/Interleukin-6; 0/Receptors, CXCR4; 0/Vascular Endothelial Growth Factor A; 143011-72-7/Granulocyte Colony-Stimulating Factor; 67256-21-7/Hepatocyte Growth Factor; EC 2.7.10.1/Proto-Oncogene Proteins c-kit; EC 2.7.10.1/Proto-Oncogene Proteins c-met
Comments/Corrections
Comment In:
Circulation. 2005 May 24;111(20):e307; author reply e307-8   [PMID:  15911713 ]
Circulation. 2004 Nov 16;110(20):3158-60   [PMID:  15545526 ]
Circulation. 2005 May 24;111(20):e307-8; author reply e307-8   [PMID:  15911712 ]

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