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Mn-Porphyrins as Novel Molecular MRI Contrast Agents.
MedLine Citation:
PMID:  22050509     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Introduction: In these experiments, we investigated a new class of therapeutic metalloporphyrins and their potential as molecular MR imaging probes for prostate cancer (PCa). Mn(III)TE-2-Pyp5+ (meso-tetrakis(N-ethyl-2-prydil)porphyrin) and Mn(III)TnHex-2-PYP5+ (meso-tetrakis(N-n-hexyl-2-pyridyl)porphyrin are powerful superoxide dismutase mimics with low toxicity and antineoplastic activity. Methods: MR imaging experiments were performed at 7.0T on a Bruker Biospec horizontal bore scanner. All in vivo experiments were performed on twelve C57 black mice implanted with RM-9 PCa cells on the hind-limb location. 10 mg/kg of MnTn-Hex-2-PyP (n=6) and 2 mg/kg MnTE-2-PyP (n=6) were administered intraperitoneally 60 and 120 minutes prior to imaging. All the images were collected using a volume coil and processed using Paravision 4.0. Results: Phantom studies reveal remarkably high T1 relaxivity changes for both hexyl and ethyl analogues, which are several-fold higher than commercially available gadolinium chelates. Observable detection limits were in the low micromolar range, 1-2 orders of magnitude lower than conventional chelates. In vivo, we readily observed MR relaxation changes in prostate tumor xenografts after a single injection of either ethyl and hexyl analogues, although T1 shortening was highest after hexyl administration. Conclusion: Following a single dose of Mn-Porphyrins, relaxation changes in prostate tumors measured 10-11 folds greater than in surrounding tissues, suggesting these probes may be particularly effective in imaging PCa foci in vivo.
Authors:
Vladimir Mouraviev; Masaki Kimura; Talaignair Venkatraman; Matvey Tsivian; Vladimira Mouravieva; Ines Batinic-Haberle; Tom James Polascik; Haichen Wang; Chris Lascola
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-3
Journal Detail:
Title:  Journal of endourology / Endourological Society     Volume:  -     ISSN:  1557-900X     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807503     Medline TA:  J Endourol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of Cincinnati, Urology/Surgery, 231 Albert Sabin Way, ML 0589, Cinicnnati, Ohio, United States, 45267, 513-558-4388, 513-558-3575; mouravvr@ucmail.uc.edu.
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