Document Detail


Mlp24 (McpX) of Vibrio cholerae implicated in pathogenicity functions as a chemoreceptor for multiple amino acids.
MedLine Citation:
PMID:  22753378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chemotaxis of Vibrio cholerae, the causative agent of cholera, has been implicated in pathogenicity. The bacterium has more than 40 genes for methyl-accepting chemotaxis protein (MCP)-like proteins (MLPs). In this study, we found that glycine and at least 18 L-amino acids, including serine, arginine, asparagine, and proline, serve as attractants to the classical biotype strain O395N1. Based on the sequence comparison with Vibrio parahaemolyticus, we speculated that at least 17 MLPs of V. cholerae may mediate chemotactic responses. Among them, Mlp24 (previously named McpX) is required for the production of cholera toxin upon mouse infection. mlp24 deletion strains of both classical and El Tor biotypes showed defects in taxis toward several amino acids, which were complemented by the expression of Mlp24. These amino acids enhanced methylation of Mlp24. Serine, arginine, asparagine, and proline were shown to bind directly to the periplasmic fragment of Mlp24. The structural information of its closest homolog, Mlp37, predicts that Mlp24 has two potential ligand-binding pockets per subunit, the membrane distal of which was suggested, by mutational analyses, to be involved in sensing of amino acids. These results suggest that Mlp24 is a chemoreceptor for multiple amino acids, including serine, arginine, and asparagine, which were previously shown to stimulate the expression of several virulence factors, implying that taxis toward a set of amino acids plays critical roles in pathogenicity of V. cholerae.
Authors:
So-ichiro Nishiyama; Daisuke Suzuki; Yasuaki Itoh; Kazuho Suzuki; Hirotaka Tajima; Akihiro Hyakutake; Michio Homma; Susan M Butler-Wu; Andrew Camilli; Ikuro Kawagishi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-02
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-15     Completed Date:  2012-10-24     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3170-8     Citation Subset:  IM    
Affiliation:
Department of Frontier Bioscience, Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Bacterial Proteins / genetics,  metabolism*
Binding Sites
Chemotaxis*
Gene Deletion
Genetic Complementation Test
Mice
Models, Molecular
Protein Conformation
Vibrio cholerae / genetics,  pathogenicity*,  physiology
Vibrio parahaemolyticus / genetics
Virulence Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
AI45746/AI/NIAID NIH HHS; R01 AI045746/AI/NIAID NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Virulence Factors
Comments/Corrections

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