Document Detail

Mixture design as first step for improved glutaminase production in solid-state fermentation by isolated Bacillus.
MedLine Citation:
PMID:  18761613     Owner:  NLM     Status:  MEDLINE    
AIM: Investigation of mixture-design impact on glutaminase production by isolated Bacillus sp. METHODS AND RESULTS: An augmented simplex centroid design was used to optimize a three (wheat bran, Bengal gram husk and palm seed fibre) component mixture for glutaminase production. Selected substrate materials showed impact on glutaminase production values at individual level by Bengal gram husk [2789 U gds(-1) (gram dry substrate] and in two-level combination with wheat bran and Bengal gram husk (maximum of 3300 U gds(-1)). CONCLUSION: Bengal gram husk is the most suitable substrate medium for glutaminase production by Bacillus sp. Maximum glutaminase production is achieved using solid-substrate mixture at two-level combinations in the ratio of 66 : 34 for Bengal gram husk and wheat bran, respectively. SIGNIFICANCE AND IMPACT OF THE STUDY: The present study has significance in large-scale production of glutaminase at commercial level with the use of multi-substrate rather than single-substrate/support material.
T Sathish; G S Lakshmi; Ch S Rao; P Brahmaiah; R S Prakasham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  47     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2009-02-25     Completed Date:  2009-03-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  256-62     Citation Subset:  IM    
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
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MeSH Terms
Bacillus / enzymology*,  growth & development,  metabolism*
Bacteriological Techniques / methods*
Biotechnology / methods*
Glutaminase / biosynthesis*
Seeds / chemistry
Reg. No./Substance:

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