Document Detail


Mitotic spindle proteomics in Chinese hamster ovary cells.
MedLine Citation:
PMID:  21647379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle from Chinese Hamster Ovary (CHO) cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology (MudPIT) analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene Ontology (GO) analysis to categorize proteins into cellular component groups. We then compared our data to the previously published CHO midbody proteome and identified proteins that are unique to the CHO spindle. Our data represent the first mitotic spindle proteome in CHO cells, which augments the list of mitotic spindle components from mammalian cells.
Authors:
Mary Kate Bonner; Daniel S Poole; Tao Xu; Ali Sarkeshik; John R Yates; Ahna R Skop
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-05-27
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-06-07     Completed Date:  2011-09-22     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e20489     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
CHO Cells
Cell Division
Cell Membrane / genetics,  metabolism
Cricetinae
Cricetulus
HeLa Cells
Humans
Microtubules / genetics,  metabolism
Mitotic Spindle Apparatus / genetics,  metabolism*
Protein Transport
Proteome / genetics,  metabolism
Proteomics*
Tandem Mass Spectrometry
Grant Support
ID/Acronym/Agency:
K01 HL092583-02/HL/NHLBI NIH HHS; K01 HL092583-03/HL/NHLBI NIH HHS; K01 HL092583-04/HL/NHLBI NIH HHS; K01 HL092583-05/HL/NHLBI NIH HHS; P41RR0118232/RR/NCRR NIH HHS; T32HG002760/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Proteome
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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