| Mitotic spindle proteomics in Chinese hamster ovary cells. | |
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MedLine Citation:
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PMID: 21647379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle from Chinese Hamster Ovary (CHO) cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology (MudPIT) analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene Ontology (GO) analysis to categorize proteins into cellular component groups. We then compared our data to the previously published CHO midbody proteome and identified proteins that are unique to the CHO spindle. Our data represent the first mitotic spindle proteome in CHO cells, which augments the list of mitotic spindle components from mammalian cells. |
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Authors:
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Mary Kate Bonner; Daniel S Poole; Tao Xu; Ali Sarkeshik; John R Yates; Ahna R Skop |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-05-27 |
Journal Detail:
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Title: PloS one Volume: 6 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2011 |
Date Detail:
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Created Date: 2011-06-07 Completed Date: 2011-09-22 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e20489 Citation Subset: IM |
Affiliation:
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Department of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals CHO Cells Cell Division Cell Membrane / genetics, metabolism Cricetinae Cricetulus HeLa Cells Humans Microtubules / genetics, metabolism Mitotic Spindle Apparatus / genetics, metabolism* Protein Transport Proteome / genetics, metabolism Proteomics* Tandem Mass Spectrometry |
| Grant Support | |
ID/Acronym/Agency:
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K01 HL092583-02/HL/NHLBI NIH HHS; K01 HL092583-03/HL/NHLBI NIH HHS; K01 HL092583-04/HL/NHLBI NIH HHS; K01 HL092583-05/HL/NHLBI NIH HHS; P41RR0118232/RR/NCRR NIH HHS; T32HG002760/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Proteome |
| Comments/Corrections | |
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