Document Detail


Mitotic activity of corneal endothelial cells in organ culture with recombinant human epidermal growth factor.
MedLine Citation:
PMID:  3494219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recombinant human epidermal growth factor (EGF) was assessed for its capacity to stimulate proliferation of human corneal endothelial cells in vitro in organ culture with 87 human corneas. The EGF in defined serum-free (S-F) media was able to stimulate endothelial cell mitosis (t test P less than 0.01) in matched transected human corneas after a four-day incubation period as judged by histologic studies. Clearly defined endothelial mitotic figures were seen in all stages of cell division throughout the endothelial cell layer. The implication of increasing corneal endothelial cell numbers in donor corneas before transplantation using a human growth factor, potentially available in pure form in unlimited quantities, is discussed.
Authors:
J M Couch; P Cullen; T A Casey; J W Fabre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ophthalmology     Volume:  94     ISSN:  0161-6420     ISO Abbreviation:  Ophthalmology     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-04-27     Completed Date:  1987-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802443     Medline TA:  Ophthalmology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Cell Division / drug effects
Child
Child, Preschool
Cornea / cytology*,  drug effects
Endothelium / cytology*,  drug effects
Epidermal Growth Factor / pharmacology*
Humans
Infant
Middle Aged
Mitosis / drug effects
Organ Culture Techniques
Recombinant Proteins / pharmacology
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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