Document Detail


Mitotic rate in melanoma: prognostic value of immunostaining and computer-assisted image analysis.
MedLine Citation:
PMID:  23629443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prognostic value of mitotic rate in melanoma is increasingly recognized, particularly in thin melanoma in which the presence or absence of a single mitosis/mm can change staging from T1a to T1b. Still, accurate mitotic rate calculation (mitoses/mm) on hematoxylin and eosin (H&E)-stained sections can be challenging. Antimonoclonal mitotic protein-2 (MPM-2) and antiphosphohistone-H3 (PHH3) are 2 antibodies reported to be more mitosis-specific than other markers of proliferation such as Ki-67. We used light microscopy and computer-assisted image analysis software to quantify MPM-2 and PHH3 staining in melanoma. We then compared mitotic rates by each method with conventional H&E-based mitotic rate for correlation with clinical outcomes. Our study included primary tissues from 190 nonconsecutive cutaneous melanoma patients who were prospectively enrolled at New York University Langone Medical Center with information on age, gender, and primary tumor characteristics. The mitotic rate was quantified manually by light microscopy of corresponding H&E-stained, MPM-2-stained, and PHH3-stained sections. Computer-assisted image analysis was then used to quantify immunolabeled mitoses on the previously examined PHH3 and MPM-2 slides. We then analyzed the association between mitotic rate and both progression-free and melanoma-specific survival. Univariate analysis of PHH3 found significant correlation between increased PHH3 mitotic rate and decreased progression-free survival (P=0.04). Computer-assisted image analysis enhanced the correlation of PHH3 mitotic rate with progression-free survival (P=0.02). Regardless of the detection method, neither MPM-2 nor PHH3 offered significant advantage over conventional H&E determination of mitotic rate.
Authors:
Christopher S Hale; Meng Qian; Michelle W Ma; Patrick Scanlon; Russell S Berman; Richard L Shapiro; Anna C Pavlick; Yongzhao Shao; David Polsky; Iman Osman; Farbod Darvishian
Related Documents :
12421663 - Event-related brain potential evidence for a response of inferior temporal cortex to fa...
19762923 - Discriminative face alignment.
12885023 - Aberro-polariscope for the human eye.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of surgical pathology     Volume:  37     ISSN:  1532-0979     ISO Abbreviation:  Am. J. Surg. Pathol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-13     Completed Date:  2013-07-18     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  7707904     Medline TA:  Am J Surg Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  882-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Disease-Free Survival
Female
Humans
Image Interpretation, Computer-Assisted / methods*
Immunohistochemistry
Kaplan-Meier Estimate
Male
Melanoma / mortality,  pathology*
Middle Aged
Mitotic Index / methods*
Neoplasm Staging / methods*
Proportional Hazards Models
Skin Neoplasms / mortality,  pathology*
Tumor Markers, Biological / analysis
Grant Support
ID/Acronym/Agency:
1 UL1 RR029893/RR/NCRR NIH HHS; 5 P30 CA016087/CA/NCI NIH HHS; P30 CA016087/CA/NCI NIH HHS; UL1 TR000038/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Tumor Markers, Biological
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  High-grade lung adenocarcinoma with fetal lung-like morphology: clinicopathologic, immunohistochemic...
Next Document:  Poor Interobserver Reproducibility in the Diagnosis of High-grade Endometrial Carcinoma.