| Mitomycin-C is an unreliable inhibitor for study of DNA synthesis in Plasmodium. | |
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MedLine Citation:
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PMID: 3095638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cytophotometric studies on DNA synthesis during asexual and sexual development of Plasmodium berghei contradicted earlier conclusions on DNA synthesis in Plasmodium which were largely based on experiments in which mitomycin-C had been used as a DNA replication inhibitor. Therefore, the effect of mitomycin on intra erythrocytic asexual development and on microgametogenesis, fertilization and zygote/ookinete development of P. berghei was studied in vitro. All DNA-synthesizing stages (schizonts, exflagellating microgametocytes and zygotes) and also DNA synthesis itself in all such stages, are totally unaffected by mitomycin concentrations 10 times higher than that which inhibits normal development of the non-DNA-synthesizing rings and trophozoites. The results are explained by the mode of action of mitomycin. |
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Authors:
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C J Janse; P F van der Klooster; H J van der Kaay; M van der Ploeg; J P Overdulve |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and biochemical parasitology Volume: 21 ISSN: 0166-6851 ISO Abbreviation: Mol. Biochem. Parasitol. Publication Date: 1986 Oct |
Date Detail:
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Created Date: 1986-12-11 Completed Date: 1986-12-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8006324 Medline TA: Mol Biochem Parasitol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 33-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytophotometry DNA / biosynthesis* Fertilization / drug effects Gametogenesis / drug effects Mitomycin Mitomycins / pharmacology* Plasmodium berghei / drug effects*, growth & development, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Mitomycins; 50-07-7/Mitomycin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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