Document Detail

Mitogenic growth factors regulate differentially early gene mRNA expression: a study on two clones of 3T3 fibroblasts.
MedLine Citation:
PMID:  1309504     Owner:  NLM     Status:  MEDLINE    
The relationship between cell proliferation and mRNA levels of the immediate early genes c-fos, c-jun, and jun B has been investigated in two clones of 3T3 fibroblasts (D1-3T3 and N2-3T3) upon treatment with basic fibroblast growth factor (bFGF), thrombin, phorbol 12-myristate 13-acetate (PMA) and dibutyryl cyclic AMP (Bt2cAMP). The 3T3-derived clone D1-3T3 almost stops dividing upon serum deprivation, while the N2-3T3 clone does not. The proliferation of the two clones was stimulated by thrombin and PMA and inhibited by Bt2cAMP. Basic FGF stimulated the growth of D1-3T3 but partly inhibited that of N2-3T3 cells. In spite of variable mitogenic response, immediate early genes, c-fos, c-jun, jun B, and c-myc, were induced by the growth factors and by PMA in both cell clones. In our experimental conditions the early gene mRNAs were expressed independently; i.e., the expression of one protooncogene had no bearing on the expression of the other. The cell growth was not directly related to the expression of a particular protooncogene mRNA. Data are presented showing that early gene mRNA expression induced by bFGF or thrombin was not mediated by protein kinase C activation while thrombin-induced mitosis was. Basic FGF induced a part of c-jun mRNA expression, but not mitosis, through a pertussis toxin-sensitive mechanism.
T Janet; G Labourdette; M Sensenbrenner; B Pettmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  198     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-02-10     Completed Date:  1992-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  305-14     Citation Subset:  IM    
Laboratoire de Neurobiologie Ontogénique, CNRS UPR 417, Strasbourg, France.
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MeSH Terms
3T3 Cells
Blotting, Northern
Bucladesine / pharmacology
Calcium / metabolism
Clone Cells
DNA Replication / drug effects*
Dose-Response Relationship, Drug
Fibroblast Growth Factor 2 / pharmacology*
Gene Expression Regulation / drug effects*
Genes, fos
Genes, jun
Pertussis Toxin
Proto-Oncogene Proteins c-fos / genetics
Proto-Oncogene Proteins c-jun / genetics
Proto-Oncogene Proteins c-myc / genetics
Tetradecanoylphorbol Acetate / pharmacology
Thrombin / pharmacology
Virulence Factors, Bordetella / pharmacology
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Proto-Oncogene Proteins c-myc; 0/Virulence Factors, Bordetella; 103107-01-3/Fibroblast Growth Factor 2; 16561-29-8/Tetradecanoylphorbol Acetate; 362-74-3/Bucladesine; 7440-70-2/Calcium; EC Toxin; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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