Document Detail


Mitogenesis by serum and PDGF is independent of PI degradation and PKC in VSMC.
MedLine Citation:
PMID:  2495729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Capacities of serum, platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) on phosphatidylinositol (PI) degradation and cell growth were compared in cultured vascular smooth muscle cells (VSMC) from rat aorta. The role of protein kinase C (PKC) in growth control was also evaluated using polymixin B, a selective inhibitor of PKC. Both dialyzed and nondialyzed fetal bovine serum (FBS) in concentrations from 2 to 20% stimulated [3H]thymidine incorporation into DNA and cell growth without producing corresponding increases in PI turnover. Moreover, both PDGF (40-160 ng/ml) and FGF (6.25-150 ng/ml) also stimulated mitogenesis, but PDGF was more effective although less potent. Mitogenic amounts of PDGF did not stimulate PI turnover, whereas a maximally mitogenic amount of FGF (50 ng/ml) did produce a slight increase. Polymixin B inhibited PKC activity (IC50, 32 microM) from these cells but failed to suppress DNA synthesis produced by 10% FBS or PDGF (50 ng/ml). However, it did suppress that by FGF (50 ng/ml). Angiotensin II (10(-11)-10(-7) M) and phorbol 12,13-dibutyrate (PDB, 1-20 nM) were not mitogenic in the presence or absence of insulin (10 micrograms/ml) or the calcium ionophore A23187 (0.25-4 microM), under serum-free conditions. Instead, PDB inhibited mitogenesis of cells maintained under 0.2% FBS or stimulated with insulin (10 micrograms/ml).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
M Kihara; P J Robinson; S H Buck; R C Dage
Related Documents :
18199429 - Antiproliferative effect of panaxynol on rasmcs via inhibition of erk1/2 and creb.
18394589 - Diadenosine polyphosphates ap3a and ap4a, but not ap5a or ap6a, induce proliferation of...
10198059 - Epigallocathechin-3 gallate selectively inhibits the pdgf-bb-induced intracellular sign...
7694289 - Protein kinase a antagonizes platelet-derived growth factor-induced signaling by mitoge...
9144559 - Regulation by camp of stat1 activation in hepatic stellate cells.
9637709 - Association and direct activation of signal transducer and activator of transcription1a...
24169049 - Pi3 kinase directly phosphorylates akt1/2 at ser473/474 in the insulin signal transduct...
20364819 - Mechanism of oxidative stress-induced ask1-catalyzed mkk6 phosphorylation.
6265579 - Studies on the corticotrophin releasing activity of a posterior pituitary extract.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  256     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-18     Completed Date:  1989-05-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C886-92     Citation Subset:  IM    
Affiliation:
Merrell Dow Research Institute, Cincinnati 45215.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Angiotensin II / pharmacology
Animals
Aorta, Thoracic
Blood*
Calcimycin / pharmacology
Cell Division
Cells, Cultured
DNA / biosynthesis
Dialysis
Fibroblast Growth Factors / pharmacology
Insulin / pharmacology
Male
Mitosis*
Muscle, Smooth, Vascular / cytology*,  metabolism
Phorbol 12,13-Dibutyrate / pharmacology
Phosphatidylinositols / metabolism*
Platelet-Derived Growth Factor / pharmacology*
Polymyxin B / pharmacology
Protein Kinase C / antagonists & inhibitors,  metabolism*
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Phosphatidylinositols; 0/Platelet-Derived Growth Factor; 11061-68-0/Insulin; 11128-99-7/Angiotensin II; 1404-26-8/Polymyxin B; 37558-16-0/Phorbol 12,13-Dibutyrate; 52665-69-7/Calcimycin; 62031-54-3/Fibroblast Growth Factors; 9007-49-2/DNA; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Aldosterone-induced proteins: purification and localization of GP65,70.
Next Document:  Influence of age, fat cell weight, and obesity on O2 consumption of human adipose tissue.