Document Detail

Mitogenesis by serum and PDGF is independent of PI degradation and PKC in VSMC.
MedLine Citation:
PMID:  2495729     Owner:  NLM     Status:  MEDLINE    
Capacities of serum, platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) on phosphatidylinositol (PI) degradation and cell growth were compared in cultured vascular smooth muscle cells (VSMC) from rat aorta. The role of protein kinase C (PKC) in growth control was also evaluated using polymixin B, a selective inhibitor of PKC. Both dialyzed and nondialyzed fetal bovine serum (FBS) in concentrations from 2 to 20% stimulated [3H]thymidine incorporation into DNA and cell growth without producing corresponding increases in PI turnover. Moreover, both PDGF (40-160 ng/ml) and FGF (6.25-150 ng/ml) also stimulated mitogenesis, but PDGF was more effective although less potent. Mitogenic amounts of PDGF did not stimulate PI turnover, whereas a maximally mitogenic amount of FGF (50 ng/ml) did produce a slight increase. Polymixin B inhibited PKC activity (IC50, 32 microM) from these cells but failed to suppress DNA synthesis produced by 10% FBS or PDGF (50 ng/ml). However, it did suppress that by FGF (50 ng/ml). Angiotensin II (10(-11)-10(-7) M) and phorbol 12,13-dibutyrate (PDB, 1-20 nM) were not mitogenic in the presence or absence of insulin (10 micrograms/ml) or the calcium ionophore A23187 (0.25-4 microM), under serum-free conditions. Instead, PDB inhibited mitogenesis of cells maintained under 0.2% FBS or stimulated with insulin (10 micrograms/ml).(ABSTRACT TRUNCATED AT 250 WORDS)
M Kihara; P J Robinson; S H Buck; R C Dage
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  256     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-18     Completed Date:  1989-05-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C886-92     Citation Subset:  IM    
Merrell Dow Research Institute, Cincinnati 45215.
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MeSH Terms
Angiotensin II / pharmacology
Aorta, Thoracic
Calcimycin / pharmacology
Cell Division
Cells, Cultured
DNA / biosynthesis
Fibroblast Growth Factors / pharmacology
Insulin / pharmacology
Muscle, Smooth, Vascular / cytology*,  metabolism
Phorbol 12,13-Dibutyrate / pharmacology
Phosphatidylinositols / metabolism*
Platelet-Derived Growth Factor / pharmacology*
Polymyxin B / pharmacology
Protein Kinase C / antagonists & inhibitors,  metabolism*
Rats, Inbred Strains
Reg. No./Substance:
0/Phosphatidylinositols; 0/Platelet-Derived Growth Factor; 11061-68-0/Insulin; 11128-99-7/Angiotensin II; 1404-26-8/Polymyxin B; 37558-16-0/Phorbol 12,13-Dibutyrate; 52665-69-7/Calcimycin; 62031-54-3/Fibroblast Growth Factors; 9007-49-2/DNA; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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