Document Detail

Mitogen-stimulated TIS21 protein interacts with a protein-kinase-Calpha-binding protein rPICK1.
MedLine Citation:
PMID:  11237868     Owner:  NLM     Status:  MEDLINE    
TIS21 is induced transiently by PMA and a number of extracellular stimuli. Yeast two-hybrid screening has identified three TIS21 interacting clones from a rat cDNA library [Lin, Gary, Yang, Clarke and Herschman (1996) J. Biol. Chem 271, 15034-15044]. The amino acid sequence deduced from clone 5A shows 96.9% identity with the murine PICK1, a protein kinase Calpha (PKCalpha)-binding protein postulated to act as an intracellular receptor for PKC. A fusion protein of glutathione S-transferase and rPICK1 associates with the TIS21 translated in vitro, suggesting a direct physical interaction between these two proteins. TIS21 and rPICK1 are co-immunoprecipitated from NIH 3T3 cells overexpressing these two proteins. This indicates that the interaction also occurs in mammalian cells. Deletion of the PDZ domain at the N-terminus of rPICK1 abolishes its interaction with TIS21. A putative carboxylate-binding loop required for PICK1 to bind PKCalpha [Staudinger, Lu and Olson (1997) J. Biol. Chem 272, 32019-32024] is within this deleted region. Our results suggest a potential competition between TIS21 and PKC for binding to PICK1. We show that recombinant TIS21 is phosphorylated by PKC in vitro. The catalytic activity of PKC towards TIS21 is significantly decreased in the presence of rPICK1, whereas phosphorylation of histone by PKC is not affected. rPICK1 seems to modulate the phosphorylation of TIS21 through specific interactions between these two proteins. TIS21 might have a role in PKC-mediated extracellular signal transduction through its interaction with rPICK1.
W J Lin; Y F Chang; W L Wang; C Y Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  354     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-05-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  635-43     Citation Subset:  IM    
Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, 112, Taiwan, Republic of China.
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MeSH Terms
3T3 Cells
Amino Acid Sequence
Carrier Proteins / genetics*,  metabolism*
Cloning, Molecular
Genes, Tumor Suppressor*
Immediate-Early Proteins / genetics,  metabolism*
Molecular Sequence Data
Nuclear Proteins / genetics,  metabolism*
Precipitin Tests
Protein Kinase C / metabolism
Protein Structure, Tertiary
Recombinant Fusion Proteins*
Sequence Deletion
Sequence Homology, Amino Acid
Tumor Suppressor Proteins
Two-Hybrid System Techniques
Reg. No./Substance:
0/Btg2 protein, mouse; 0/Carrier Proteins; 0/Immediate-Early Proteins; 0/Nuclear Proteins; 0/Prkcabp protein, mouse; 0/Prkcabp protein, rat; 0/Recombinant Fusion Proteins; 0/Tumor Suppressor Proteins; EC Kinase C

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