| Mitochondrial superoxide anion radicals mediate induction of apoptosis in cardiac myoblasts exposed to chronic hypoxia. | |
| | |
MedLine Citation:
|
PMID: 20971059 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Both reactive oxygen species (ROS) and ATP depletion may be significant in hypoxia-induced damage and death, either collectively or independently, with high energy requiring, metabolically active cells being the most susceptible to damage. We investigated the kinetics and effects of ROS production in cardiac myoblasts, H9C2 cells, under 2%, 10% and 21% O₂ in the presence or absence of apocynin, rotenone and carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone. H9C2 cells showed significant loss of viability within 30 min of culture at 2% oxygen which was not due to apoptosis, but was associated with an increase in protein oxidation. However, after 4 h, apoptosis induction was observed at 2% oxygen and also to a lesser extent at 10% oxygen; this was dependent on the levels of mitochondrial superoxide anion radicals determined using dihydroethidine. Hypoxia-induced ROS production and cell death could be rescued by the mitochondrial complex I inhibitor, rotenone, despite further depletion of ATP. In conclusion, a change to superoxide anion radical steady state level was not detectable after 30 min but was evident after 4 h of mild or severe hypoxia. Superoxide anion radicals from the mitochondrion and not ATP depletion is the major cause of apoptotic cell death in cardiac myoblasts under chronic, severe hypoxia. |
| | |
Authors:
|
Rajitha T Kolamunne; Michelle Clare; Helen R Griffiths |
Related Documents
:
|
19433269 - Cadmium-induced apoptosis is mediated by the translocation of aif to the nucleus in rat... 20600869 - Radical changes in multiple sclerosis pathogenesis. 10066639 - Oxidant-induced lung injury in anticancer therapy. 20146889 - Oxidative stress and apoptosis of human brain microvascular endothelial cells induced b... 20500659 - Peroxiredoxin: a central player in immune modulation. 10554089 - Therapeutic targets for hypoxia-elicited pathways. 12473019 - Cytokines and cognition--the case for a head-to-toe inflammatory paradigm. 21551229 - The racgap arhgap15 is a master negative regulator of neutrophil functions. 18157869 - Ceramide inhibits development and cytokinesis and induces apoptosis in preimplantation ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-29 |
Journal Detail:
|
Title: Archives of biochemistry and biophysics Volume: 505 ISSN: 1096-0384 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-01-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
|
Languages: eng Pagination: 256-65 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Aston University, Birmingham, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rearrangement reactions catalyzed by cytochrome P450s.
Next Document: Trichosanthin affects HSV-1 replication in Hep-2 cells.