Document Detail


Mitochondrial respiration on rumenic and linoleic acids.
MedLine Citation:
PMID:  11356175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rumenic acid (cis-9, trans-11-C(18:2)) represents approx. 80% of conjugated linoleic acid (CLA) in dairy products. CLA has been shown to exert beneficial effects on health, but little work has been devoted to the ability to oxidize CLA isomers and the role of these isomers in the modulation of beta-oxidation flux. In the present study, respiration on rumenic acid was compared with that on linoleic acid (cis-9, cis-12-C(18:2)) with the use of rat liver mitochondria. In state-3, respiration was decreased by half with rumenic acid in comparison with linoleic acid. In the uncoupled state, respiration on CLA remained 30% lower. The lower ability to oxidize CLA was investigated through characterization of the enzymic steps. Rumenic acid was 33% less activated by acyl-CoA synthase than was linoleic acid. However, after such activation, the transfer of both acyl moieties to carnitine by carnitine acyltransferase I (CAT I) was of the same order. Moreover, CAT II activity was comparable with either isomer. After prior incubation with rumenic acid, oxidation of octanoic acid by re-isolated mitochondria was unimpaired, but that of palmitoleic acid was impaired unless linoleic acid was used in the prior incubation. The slower respiration on cis-9, trans-11-C(18:2) is suggested to arise from lower carnitine-acylcarnitine translocase activity towards the acylcarnitine form, causing an upstream increase in the corresponding acyl-CoA.
Authors:
P Clouet; L Demizieux; J Gresti; P Degrace
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  29     ISSN:  0300-5127     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-17     Completed Date:  2001-09-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  320-5     Citation Subset:  IM    
Affiliation:
UPRES E.A. 2422, Faculté des Sciences Gabriel, Université de Bourgogne, 21000 Dijon, France. pclouet@u-bourgogne.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Carnitine Acyltransferases / metabolism
Cattle
Cell Respiration*
Coenzyme A Ligases / metabolism
Dairy Products
Fatty Acids, Monounsaturated / metabolism
Linoleic Acid / chemistry,  metabolism*
Liver / cytology,  metabolism
Mitochondria, Liver / metabolism*
Octanoic Acids / metabolism
Oxygen Consumption
Rats
Stearic Acids / metabolism
Stereoisomerism
Chemical
Reg. No./Substance:
0/Fatty Acids, Monounsaturated; 0/Octanoic Acids; 0/Stearic Acids; 124-07-2/caprylic acid; 2091-29-4/palmitoleic acid; 2197-37-7/Linoleic Acid; 57-11-4/stearic acid; EC 2.3.1.-/Carnitine Acyltransferases; EC 6.2.1.-/Coenzyme A Ligases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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