Document Detail


Mitochondrial regulation of cell cycle progression during development as revealed by the tenured mutation in Drosophila.
MedLine Citation:
PMID:  16326395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The precise control of the cell cycle requires regulation by many intrinsic and extrinsic factors. Whether the metabolic status of the cell exerts a direct control over cell cycle checkpoints is not well understood. We isolated a mutation, tenured (tend), in a gene encoding cytochrome oxidase subunit Va. This mutation causes a drop in intracellular ATP to levels sufficient to maintain cell survival, growth, and differentiation, but not to enable progression through the cell cycle. Analysis of this gene in vivo and in cell lines shows that a specific pathway involving AMPK and p53 is activated that causes elimination of Cyclin E, resulting in cell cycle arrest. We demonstrate that in multiple tissues the mitochondrion has a direct and specific role in enforcing a G1-S cell cycle checkpoint during periods of energy deprivation.
Authors:
Sudip Mandal; Preeta Guptan; Edward Owusu-Ansah; Utpal Banerjee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Developmental cell     Volume:  9     ISSN:  1534-5807     ISO Abbreviation:  Dev. Cell     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-05     Completed Date:  2006-01-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  843-54     Citation Subset:  IM    
Affiliation:
Department of Molecular, Cell, and Developmental Biology, Department of Biological Chemistry and Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USA.
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MeSH Terms
Descriptor/Qualifier:
AMP-Activated Protein Kinases
Adenosine Triphosphate / metabolism
Animals
Animals, Genetically Modified
Cell Differentiation
Cell Proliferation
Cell Survival
Cyclin E / metabolism
Drosophila melanogaster / genetics,  growth & development*,  metabolism
Electron Transport Complex IV / genetics*,  metabolism
Female
G1 Phase*
Male
Mitochondria / metabolism*
Multienzyme Complexes / metabolism
Mutation*
Protein-Serine-Threonine Kinases / metabolism
S Phase*
Tumor Suppressor Protein p53 / metabolism
Grant Support
ID/Acronym/Agency:
R01-EY08152/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Cyclin E; 0/Multienzyme Complexes; 0/Tumor Suppressor Protein p53; 56-65-5/Adenosine Triphosphate; EC 1.9.3.1/Electron Transport Complex IV; EC 2.7.11.1/AMP-Activated Protein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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