Document Detail


Mitochondrial reactive oxygen species originating from Romo1 exert an important role in normal cell cycle progression by regulating p27(Kip1) expression.
MedLine Citation:
PMID:  19513905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reactive oxygen species (ROS) steady-state levels are required for entry into the S phase of the cell cycle in normal cells, as well as in tumour cells. However, the contribution of mitochondrial ROS to normal cell proliferation has not been well investigated thus far. A previous report showed that Romo1 was responsible for the high ROS levels in tumour cells. Here, we show that endogenous ROS generated by Romo1 are indispensable for cell cycle transition from G1 to S phase in normal WI-38 human lung fibroblasts. The ROS level in these cells was down-regulated by Romo1 knockdown, resulting in cell cycle arrest in the G1 phase. This arrest was associated with an increase in the level of p27(Kip1). These results demonstrate that mitochondrial ROS generated by Romo1 expression is required for normal cell proliferation and it is suggested that Romo1 plays an important role in redox signalling during normal cell proliferation.
Authors:
Jin Sil Chung; Seung Baek Lee; Seon Ho Park; Sung Tae Kang; Ah Ram Na; Tong-Shin Chang; Hyung Jung Kim; Young Do Yoo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  43     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2010-01-19     Completed Date:  2010-03-22     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  729-37     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Cell Biology, Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul, 136-705, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / physiology*
Cell Division
Cell Line
Cyclin-Dependent Kinase Inhibitor p27 / biosynthesis,  genetics,  physiology*
Fibroblasts / metabolism*
Gene Expression Regulation / physiology*
Gene Knockdown Techniques
Humans
Membrane Proteins / antagonists & inhibitors,  genetics,  physiology*
Mitochondria / metabolism*
Mitochondrial Proteins / antagonists & inhibitors,  genetics,  physiology*
RNA, Small Interfering / pharmacology
Reactive Oxygen Species / metabolism*
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Mitochondrial Proteins; 0/RNA, Small Interfering; 0/ROMO1 protein, human; 0/Reactive Oxygen Species; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27

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