Document Detail


Mitochondrial profiling of dog hairs.
MedLine Citation:
PMID:  12787657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to make greater use of dog hairs as forensic evidence, we have developed a robust method for duplex amplification of adjacent 306 and 332bp amplicons within the 5' hypervariable region (5' HVR) of the canine mitochondrial control region. In support of this, a 595bp region covering 35 polymorphic sites has been sequenced from the blood of 105 UK dogs. In total, 30 different haplotypes were observed, 13 only once whilst the commonest was seen 14 times; the overall exclusion capacity is 0.929. One animal was heteroplasmic in blood for a single base deletion and showed phenotypes ranging from near complete deletion to a predominance of the base among a sample of 12 hairs. In contrast, no evidence of heteroplasmy was seen in single hairs from 20 dogs which were not visibly heteroplasmic in blood. Phylogenetic analysis and comparisons with other published databases highlighted instances of possible recurrent mutation which may be relevant when interpreting single base differences between samples.
Authors:
Jon H Wetton; Jenny E Higgs; Adrian C Spriggs; Chris A Roney; Carol S F Tsang; Aiden P Foster
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Forensic science international     Volume:  133     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2003-08-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  235-41     Citation Subset:  IM    
Affiliation:
Forensic Science Service, R&D, Trident Court, Birmingham Business Park, Solihull B37 7YN, UK. jwe02@fss.org.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Mitochondrial / analysis*
Databases as Topic
Dogs / genetics*
Hair / chemistry*
Haplotypes
Mutation
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Analysis, DNA*
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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