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Mitochondrial pathway of apoptosis in the hepatopancreas of the freshwater crab Sinopotamon yangtsekiense exposed to cadmium.
MedLine Citation:
PMID:  21831345     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cadmium (Cd) is one of the most common toxic metals in water. To investigate the mechanism of Cd-induced apoptosis in the hepatopancreas, freshwater crabs Sinopotamon yangtsekiense were exposed to 0, 3.56, 7.12, 14.25, 28.49 and 56.98mg/L Cd for 48h. After a 48h exposure, apoptosis and necroptosis were apparent in the group exposed to 28.49mg/L Cd and only one case of necrosis was observed in the highest concentration of Cd. Electronic microscopy revealed chromatin condensation under nuclear membrane and mitochondrial membrane rupture in 14.25 and 28.49mg/L Cd treatment groups. Brown colored apoptotic cells were detected with the TUNEL test in all Cd-treatment groups. The AI in 56.98mg/L group was 1.4-fold greater than that in crabs exposed to 14.25mg/L Cd. Caspase-9, caspase-3, SDH and Ca(2+)-ATPase activities increased with increasing Cd concentration. However, the activities of caspase-8 and LDH did not change significantly compared with control group. These results implied that Cd induced apoptosis in the hepatopancreas occurs through a mitochondrial pathway.
Authors:
Dongmei Liu; Bo Yan; Jian Yang; Wenwen Lei; Lan Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-23
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  105     ISSN:  1879-1514     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-8-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  394-402     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Laboratory of the Bio-effect and Molecular Mechanism on Classical Environmental Pollutants, School of Life Science, Shanxi University, 96 Wucheng Road, Taiyuan 030006, Shanxi Province, People's Republic of China.
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